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PXD037741-1

PXD037741 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMitochondrial dysfunction rapidly modulates the abundance and thermal stability of cellular proteins
DescriptionCellular functionality relies on a well-balanced, but highly dynamic proteome. Dysfunctionof mitochondrial protein import leads to the cytosolic accumulation of mitochondrial precursor proteins which compromise cellular proteostasis and trigger the mitoprotein-induced stress response. To dissect the effects of mitochondrial dysfunction on the cellular proteome as a whole, we developed pre-post thermal proteome profiling (ppTPP). This multiplexed time-resolved proteome-wide thermal stability profiling approach with isobaric peptide tags in combination with a pulse SILAC labeling elucidated dynamic proteostasis changes in several dimensions: In addition to adaptations in protein abundance, we observed rapid modulations of the thermal stability of individual cellular proteins. Strikingly, different functional groups of proteins showed characteristic response patterns and reacted with group-specific kinetics, allowing the identification of the functional modules that are relevant for mitoprotein-induced stress. Thus, our new ppTPP approach uncovered a complex response network that orchestrates proteome homeostasis in eukaryotic cells by time-controlled adaptations of protein abundance and protein stability.
HostingRepositoryPRIDE
AnnounceDate2023-02-21
AnnouncementXMLSubmission_2023-02-21_09:54:55.803.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFrankStein
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-10-26 15:46:35ID requested
12023-02-21 09:54:56announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Mitochondria / Proteasome / Protein folding / Proteostasis / Thermal proteome profiling / Stress response
Contact List
Johannes M.Herrmann
contact affiliationUniveristy of Kaiserslautern Cell Biology
contact emailhannes.herrmann@bio.uni-kl.de
lab head
FrankStein
contact affiliationEMBL
contact emailfrank.stein@embl.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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