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PXD037736-1

PXD037736 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCoupled induction of prophage and virulence factors during tick transmission of the Lyme disease spirochete
DescriptionThe alternative sigma factor RpoS plays a central role in the critical host-adaptive response of the Lyme disease spirochete, Borrelia burgdorferi. We previously identified bbd18 as a negative regulator of RpoS but could not inactivate bbd18 in wild-type spirochetes. In the current study we employed an inducible bbd18 gene to demonstrate the essential nature of BBD18 for viability of wild-type spirochete viability in vitro and at a unique point in vivo. Transcriptomic analyses of BBD18 depleted cells demonstrated global induction of RpoS-dependent genes prior to lysis, with the absolute requirement for BBD18, both in vitro and in vivo, circumvented by deletion of rpoS. The increased expression of plasmid prophage genes and the presence of phage particles in the supernatants of lysing cultures indicate that RpoS regulates phage lysis-lysogeny decisions. Through this work we identify a mechanistic link between endogenous transducing prophages and the RpoS-dependent adaptive response of the Lyme disease spirochete. The alternative sigma factor RpoS plays a central role in the critical host-adaptive response of the Lyme disease spirochete, Borrelia burgdorferi. We previously identified bbd18 as a negative regulator of RpoS but could not inactivate bbd18 in wild-type spirochetes. In the current study we employed an inducible bbd18 gene to demonstrate the essential nature of BBD18 for viability of wild-type spirochete viability in vitro and at a unique point in vivo. Transcriptomic analyses of BBD18 depleted cells demonstrated global induction of RpoS-dependent genes prior to lysis, with the absolute requirement for BBD18, both in vitro and in vivo, circumvented by deletion of rpoS. The increased expression of plasmid prophage genes and the presence of phage particles in the supernatants of lysing cultures indicate that RpoS regulates phage lysis-lysogeny decisions. Through this work we identify a mechanistic link between endogenous transducing prophages and the RpoS-dependent adaptive response of the Lyme disease spirochete.
HostingRepositoryPRIDE
AnnounceDate2022-12-12
AnnouncementXMLSubmission_2022-12-12_09:22:33.131.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJennyWachter
SpeciesList scientific name: Borrelia burgdorferi B31; NCBI TaxID: 224326;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-10-26 01:33:27ID requested
12022-12-12 09:22:33announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Borrelia burgdorferi, bbd18, rpoS
Contact List
PatriciaRosa
contact affiliationMolecular Genetics Section Laboratory of Bacteriology Rocky Mountain Laboratories, DIR, NIAID, NIH USA
contact emailprosa@niaid.nih.gov
lab head
JennyWachter
contact affiliationVaccine and Infectious Disease Organization
contact emailjenny.wachter@usask.ca
dataset submitter
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