PXD037703 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A degradation motif in STAU1 defines a novel family of proteins involved in inflammation |
Description | Cancer development is regulated by inflammation. Staufen 1 (STAU1) is an RNA-binding protein whose expression level is critical in cancer cells as it is related to cell proliferation or cell death. STAU1 expression levels are downregulated during mitosis due to its degradation by the E3 ubiquitin ligase anaphase-promoting complex/cyclosome (APC/C). In this paper, we map the molecular determinant involved in STAU1 degradation in mitosis to amino acids 38-50 and, by alanine scanning, we shorten the motif to F39PxPxxLxxxxL50 (FPL-motif). Mutation of the FPL-motif prevents STAU1 degradation by APC/C. Interestingly, a search in databases reveals that the FPL-motif is shared by 15 additional proteins most of them being involved in inflammation. We show that one of these proteins, MAP4K1, is indeed degraded via the FPL-motif; however, it is not a target of APC/C. Using proximity labeling with STAU1, we identify TRIM25, an E3-ubiquitin ligase involved in the innate immune response and interferon production, as responsible for STAU1 and MAP4K1 degradation, dependent on the FPL-motif. These results are consistent with previous studies that linked STAU1 to cancer-induced inflammation and identify a novel degradation motif that likely coordinates a novel family of proteins involved in inflammation. |
HostingRepository | PRIDE |
AnnounceDate | 2023-02-21 |
AnnouncementXML | Submission_2023-02-21_09:30:46.741.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD037703 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | EricBonneil |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue; acetylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | LTQ FT |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-10-25 04:39:34 | ID requested | |
⏵ 1 | 2023-02-21 09:30:47 | announced | |
2 | 2023-11-14 07:30:33 | announced | 2023-11-14: Updated project metadata. |
Publication List
10.6019/PXD037703; |
Gonzalez Quesada Y, DesGroseillers L, A Degradation Motif in STAU1 Defines a Novel Family of Proteins Involved in Inflammation. Int J Mol Sci, 23(19):(2022) [pubmed] |
Keyword List
submitter keyword: Staufen 1 |
human |
inflammation |
protein degradation |
E3-ubiquitin ligase |
degron |
TRIM25 |
Contact List
LucDesgroseillers |
contact affiliation | Université de Montréal |
contact email | luc.desgroseillers@umontreal.ca |
lab head | |
EricBonneil |
contact affiliation | Proteomic Platform |
contact email | eric.bonneil@umontreal.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD037703
- Label: PRIDE project
- Name: A degradation motif in STAU1 defines a novel family of proteins involved in inflammation