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PXD037611-1

PXD037611 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAmmonium Sulfate-based Prefractionation Improved Carbonylated Proteome Coverage in Arabidopsis thaliana Leaf Extract
DescriptionThe hypothesis that ammonium sulfate-based protein precipitation can help prefractionate the total protein extract into fractions of reduced complexity and improve the detection of low-abundance proteins was investigated. This dataset resulted from the experiments that we performed to improve the detection of the carbonylated proteins in leaf extract. A total of 122 carbonylated proteins were identified, of which 77 were found in the ammonium sulfate fractions only. This prefractionation method allowed to identify 63% more carbonylated proteins compared to the number of carbonylated proteins identified from the total crude extract without prefractionation. Our results thus showed that a simple offline prefractionation of the proteome by ammonium sulfate prior to liquid chromatography-tandem mass spectrometry significantly improved the detection of carbonylated proteins in Arabidopsis leaf extract.
HostingRepositoryMassIVE
AnnounceDate2023-02-19
AnnouncementXMLSubmission_2023-02-19_10:44:13.059.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterTagnon Missihoun
SpeciesList scientific name: Arabidopsis thaliana; common name: thale cress; NCBI TaxID: 3702;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-10-20 11:25:25ID requested
12023-02-19 10:44:13announced
Publication List
no publication
Keyword List
submitter keyword: Aldehyde reactive probe, peptide level enrichment, protein carbonylation, proteome offline prefractionation
Contact List
Tagnon Missihoun
contact affiliationUniversity of Quebec Trois-Rivieres
contact emailTagnon.Missihoun@uqtr.ca
lab head
Tagnon Missihoun
contact affiliationUniversity of Quebec Trois-Rivieres
contact emailTagnon.Missihoun@uqtr.ca
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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