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PXD037525-1

PXD037525 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSite-Selective Photocatalytic Functionalization of Peptides and Proteins at Selenocysteine
DescriptionThe importance of modified peptides and proteins for applications in drug discovery, and for illuminating biological processes at the molecular level, has fueled a demand for efficient methods that facilitate the precise modification of these biomolecules. Herein, we describe the development of a photocatalytic method for the rapid and efficient dimerization and sitespecific functionalization of peptide and protein diselenides. This methodology, dubbed the photocatalytic diselenide contraction (PDC), involves irradiation at 450 nm in the presence of an iridium photocatalyst and a phosphine and results in rapid and clean conversion of diselenides to reductively stable selenoethers. A mechanism for this unprecedented photocatalytic transformation is proposed, which is supported by photoluminescence spectroscopy and density functional theory calculations. The utility of the PDC transformation is highlighted through the dimerization of selenopeptides, and by the generation of two families of protein conjugates via the site-selective modification of calmodulin containing the 21st amino acid selenocysteine, and the C-terminal modification of a ubiquitin diselenide.
HostingRepositoryPRIDE
AnnounceDate2022-10-25
AnnouncementXMLSubmission_2022-10-25_10:03:38.738.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMarkLarance
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-10-18 08:02:09ID requested
12022-10-25 10:03:39announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Selenocysteine, modification
Contact List
MarkLarance
contact affiliationThe University of Sydney
contact emailmark.larance@sydney.edu.au
lab head
MarkLarance
contact affiliationThe University of Sydney
contact emailmark.larance@sydney.edu.au
dataset submitter
Full Dataset Link List
Dataset FTP location
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