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PXD037510-1

PXD037510 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLoss of N-terminal acetyltransferase A activity induces thermally unstable ribosomal proteins and increases their turnover
DescriptionProtein N-terminal (Nt) acetylation is one of the most abundant modifications in eukaryotes, covering ~50-80 % of the proteome, depending on species. Cells with defective Nt-acetylation display a wide array of phenotypes such as impaired growth, mating defects and increased stress sensitivity. However, the pleiotropic nature of these effects has hampered our understanding of the functional impact of protein Nt-acetylation. The main enzyme responsible for Nt-acetylation throughout the eukaryotic kingdom is the N-terminal acetyltransferase NatA. Here we employed a multi-dimensional proteomics approach to analyze Saccharomyces cerevisiae lacking NatA activity, which caused global proteome remodeling. Pulsed-SILAC experiments revealed that NatA-deficient strains consistently increased degradation of ribosomal proteins compared to wild type. Explaining this phenomenon, thermal proteome profiling uncovered decreased thermostability of ribosomes in NatA-knockouts. Our data are in agreement with a role for Nt-acetylation in promoting stability for parts of the proteome by enhancing the avidity of protein-protein interactions and folding.
HostingRepositoryPRIDE
AnnounceDate2023-10-25
AnnouncementXMLSubmission_2023-10-24_19:22:58.798.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterUlisesH Guzman
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListubiquitination signature dipeptidyl lysine; acetylated residue
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-10-17 03:26:00ID requested
12023-10-24 19:22:59announced
22023-11-14 09:12:44announced2023-11-14: Updated project metadata.
Publication List
Guzman UH, Aksnes H, Ree R, Krogh N, Jakobsson ME, Jensen LJ, Arnesen T, Olsen JV, Loss of N-terminal acetyltransferase A activity induces thermally unstable ribosomal proteins and increases their turnover in Saccharomyces cerevisiae. Nat Commun, 14(1):4517(2023) [pubmed]
Keyword List
submitter keyword: Sacharomyces cerevisiae , TPP,Nt-acetylation, PSilac
Contact List
Jesper V.Olsen
contact affiliationNovo Nordisk Foundation Center for Protein Research, Proteomics Program, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
contact emailjesper.olsen@cpr.ku.dk
lab head
UlisesH Guzman
contact affiliationNovo Nordisk Foundation Center for Protein Research
contact emailulysseshrdz@gmail.com
dataset submitter
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Dataset FTP location
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