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PXD037509-1

PXD037509 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleComplete integration of carbene-transfer chemistry into biosynthesis
DescriptionBiosynthesis is an environmentally friendly and renewable way to synthesize a broad range of natural and some new-to-nature products; however, it lacks many of the reactions and flexibility of synthetic chemistry, an example being carbene mediated reactions. While it was recently shown that these reactions can be imported into the cell, carbene donors and unnatural cofactors needed to be added exogenously to the cells, precluding cost-effective scale-up of the reaction. Here we identified a biosynthetic gene cluster that when expressed in Streptomyces albus will produce the diazo compound azaserine, which we also demonstrated can serve as a carbene donor across a carbon-carbon double bond in another intracellularly produced molecule, styrene, thereby producing a cyclopropanated product. The reaction was catalyzed with P450 mutants harboring a native cofactor with excellent diastereoselectivity and moderate yield. This work establishes a platform for introducing carbene mediated reactions into microbes for bio-manufacture and contributes to sustainable green chemistry.
HostingRepositoryPRIDE
AnnounceDate2023-05-03
AnnouncementXMLSubmission_2023-05-03_09:55:58.277.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterChristopherPetzold
SpeciesList scientific name: Streptomyces albus J1074; NCBI TaxID: 457425;
ModificationListmonohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-10-17 01:54:26ID requested
12023-05-03 09:55:58announced
22023-11-14 08:58:18announced2023-11-14: Updated project metadata.
Publication List
Keyword List
submitter keyword: streptomyces albus, P450,Biosynthesis, Carbene meidated reaction
Contact List
ChristopherPetzold
contact affiliationStaff Scientist Biological Systems & Engineering Division Lawrence Berkeley National Laboratory Berkeley CA 94720
contact emailcjpetzold@lbl.gov
lab head
ChristopherPetzold
contact affiliationLawrence Berkeley National Laboratory
contact emailcjpetzold@lbl.gov
dataset submitter
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Dataset FTP location
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