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PXD037498 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA Tribrid Hyperplexing approach for Large-scale Quantitative Proteomic Analysis
DescriptionIsobaric labeling has emerged as an indispensable quantitative proteomic approach for its unprecedented multiplexing capacity in a single analysis. Currently, different hyperplexing approaches have been developed to meet the need for increasing sample numbers in large-scale cohort analysis. In this report, we present a tribrid hyperplexing approach by the combinational use of three types of isobaric reagents, a novel isobaric tag 16-plex (IBT16) reagent, and the widely used TMT (TMT11) and TMTpro (TMT18) reagents. After the determination of the labeling efficiency and the optimization testing conditions, we systematically evaluated the identification and quantification performance of the three labeling methods in both independent and combinatorial means using the mixtures of E. coli and HeLa peptides with different ratios. Our results reveal that the three reagents are quite similar in all testing aspects although with some differences, and the combination use of three reagents to expand the multiplexing capacity is feasible. Furthermore, we provide practical recommendations for the choice of isobaric reagent combinations according to different plexes.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; S-carboxamidoethyl-L-cysteine; monohydroxylated residue
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-10-17 01:39:11ID requested
12023-03-17 06:57:19announced
Publication List
Wu Z, Xiang W, Huang L, Li S, Zhang X, Hyperplexing Approaches for up to 45-Plex Quantitative Proteomic Analysis. Anal Chem, ():(2023) [pubmed]
Keyword List
submitter keyword: TMTpro, IBT, TMT,Hyperplexing, quantitative proteomics
Contact List
contact affiliationState Key Laboratory of Genetic Engineering, Department of Biochemistry and Biophysics, School of Life Sciences, Fudan University, Shanghai 200438, China
contact emailxumin_zhang@fudan.edu.cn
lab head
contact affiliationState Key Laboratory of Genetic Engineering, Department of Biochemistry, School of Life Sciences, Fudan University
contact emailwuz16@fudan.edu.cn
dataset submitter
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Dataset FTP location
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