PXD037498-1

PXD037498 is an original dataset announced via ProteomeXchange.

Title	A Tribrid Hyperplexing approach for Large-scale Quantitative Proteomic Analysis
Description	Isobaric labeling has emerged as an indispensable quantitative proteomic approach for its unprecedented multiplexing capacity in a single analysis. Currently, different hyperplexing approaches have been developed to meet the need for increasing sample numbers in large-scale cohort analysis. In this report, we present a tribrid hyperplexing approach by the combinational use of three types of isobaric reagents, a novel isobaric tag 16-plex (IBT16) reagent, and the widely used TMT (TMT11) and TMTpro (TMT18) reagents. After the determination of the labeling efficiency and the optimization testing conditions, we systematically evaluated the identification and quantification performance of the three labeling methods in both independent and combinatorial means using the mixtures of E. coli and HeLa peptides with different ratios. Our results reveal that the three reagents are quite similar in all testing aspects although with some differences, and the combination use of three reagents to expand the multiplexing capacity is feasible. Furthermore, we provide practical recommendations for the choice of isobaric reagent combinations according to different plexes.
HostingRepository	PRIDE
AnnounceDate	2023-03-17
AnnouncementXML	Submission_2023-03-17_06:57:19.029.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	ZhenWu
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; S-carboxamidoethyl-L-cysteine; monohydroxylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-17 01:39:11	ID requested
⏵ 1	2023-03-17 06:57:19	announced

Wu Z, Xiang W, Huang L, Li S, Zhang X, Hyperplexing Approaches for up to 45-Plex Quantitative Proteomic Analysis. Anal Chem, ():(2023) [pubmed]

submitter keyword: TMTpro, IBT, TMT,Hyperplexing, quantitative proteomics

XuminZhang
contact affiliation	State Key Laboratory of Genetic Engineering, Department of Biochemistry and Biophysics, School of Life Sciences, Fudan University, Shanghai 200438, China
contact email	xumin_zhang@fudan.edu.cn
lab head
ZhenWu
contact affiliation	State Key Laboratory of Genetic Engineering, Department of Biochemistry, School of Life Sciences, Fudan University
contact email	wuz16@fudan.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037498
     1. Label: PRIDE project
     2. Name: A Tribrid Hyperplexing approach for Large-scale Quantitative Proteomic Analysis