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PXD037490-1

PXD037490 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSynaptic vesicle proteins and ATG9A self-organize in distinct vesicle phases within synapsin condensates
DescriptionEctopic expression in fibroblasts of synapsin 1 and synaptophysin is sufficient to generate condensates of vesicles highly reminiscent of synaptic vesicle (SV) clusters and with liquid-like properties. We show that unlike synaptophysin, other major integral SV membrane proteins fail to form condensates with synapsin, but coassemble into the clusters formed by synaptophysin and synapsin in this ectopic expression system. Another vesicle membrane protein, ATG9A, undergoes activity-dependent exo-endocytosis at synapses, raising questions about the relation of ATG9A traffic to the traffic of SVs. We have found that both in fibroblasts and in nerve terminals ATG9A does not coassemble into synaptophysin-positive vesicle condensates but localizes on a distinct class of vesicles that also assembles with synapsin but into a distinct phase. Our findings suggest that ATG9A undergoes differential sorting relative to SV proteins and also point to a dual role of synapsin in controlling clustering at synapses of SVs and ATG9A vesicles
HostingRepositoryPRIDE
AnnounceDate2022-12-24
AnnouncementXMLSubmission_2022-12-23_19:44:37.086.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterZon WengLai
SpeciesList scientific name: Cercopithecus aethiops (Green monkey) (Grivet); NCBI TaxID: 9534;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-10-17 01:28:18ID requested
12022-12-23 19:44:37announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: synaptophysin, synapsin, ATG9A, sypnatic vesicle
Contact List
Pietro DeCamilli
contact affiliationYale University School of Medicine
contact emailpietro.decamilli@yale.edu
lab head
Zon WengLai
contact affiliationHarvard T. H. Chan School of Public Health
contact emailzlai@hsph.harvard.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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