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PXD037423-1

PXD037423 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCharacterization of Plasma Membrane Proteins in Stylosanthes Leaves and Roots Using Simplified Enrichment Method
DescriptionPlant plasma membranes (PMs) play an important role in maintaining the stability of the intracellular environment and exchanging information with the external environment. Therefore, deciphering dynamics of PM proteome provides crucial information for elucidating cellular regulation in response to diverse stimuli. In the study, we developed a simplified method for enriching PM proteins in leaf and root tissues of a tropical forage Stylosanthes by combining differential centrifugation and Brij-58 treatment. Both immunoblot analysis and mass spectrometry demonstrated that the representation and abundance of PM proteins were increased in the enrichment fraction, and the contamination of other organellar proteins was decreased. A total of 425 and 387 proteins were predicted to be PM proteins in leaves and roots respectively. Functional analysis classified these PM proteins into six major categories (transporter, enzyme, receptor, membrane structure protein, vesicular trafficking and chaperone), and orthologs of many PM proteins regulating the responses to abiotic and biotic stresses have been detected. In addition, the PM localization of a newly identified receptor-like kinase, SgRKL1, has been verified. Together, these results show that the simplified PM enrichment method can be successfully applied to different plant tissue types and to study the dynamics of PM proteome of Stylosanthes in response to multiple stresses.
HostingRepositoryPRIDE
AnnounceDate2022-12-14
AnnouncementXMLSubmission_2022-12-14_05:02:51.754.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitter丽云杨
SpeciesList scientific name: Stylosanthes guianensis; NCBI TaxID: 62615;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-10-14 05:54:21ID requested
12022-12-14 05:02:52announced
22023-11-14 08:59:11announced2023-11-14: Updated project metadata.
Publication List
Keyword List
submitter keyword: proteomics,plasma membrane, Brij-58 treatment, protein enrichment, Stylosanthes
Contact List
yang liyun
contact affiliationHainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, College of Tropical Crops, Hainan University, Haikou, China
contact email1058200235@qq.com
lab head
丽云杨
contact affiliation海南大学
contact email1058200235@qq.com
dataset submitter
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Dataset FTP location
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