PXD037392

PXD037392 is an original dataset announced via ProteomeXchange.

Title	Peripheral proteomic changes after electroconvulsive seizures (ECS) in a rodent model of non-respons to chronic fluoxetine
Description	Major depressive disorder (MDD) is the psychiatric disorder with the highest prevalence in the world. Pharmacological antidepressant treatment (AD), such as selective serotonin reuptake inhibitors [SSRI, i.e. fluoxetine (Flx)] is the first line of treatment for MDD. Despite its efficacy, lack of AD response occurs in numerous patients characterizing Difficult-to-treat Depression. ElectroConvulsive Therapy (ECT) is a highly effective treatment inducing rapid improvement in depressive symptoms and high remission rates of ~50–63% in patients with pharmaco-resistant depression. Nevertheless, the need to develop reliable treatment response predictors to guide personalized AD strategies and supplement clinical observation is becoming a pressing clinical objective. Here, we propose to establish a proteomic peripheral biomarkers signature of ECT response in an anxio/depressive animal model of non-response to AD. Using an emotionality score based on the analysis complementary behavioral tests of anxiety/depression (Elevated Plus Maze, Novelty Suppressed Feeding, Splash Test), we showed that a 4-week corticosterone treatment (35 μg/ml, Cort model) in C57BL/6JRj male mice induced an anxiety/depressive-like behavior. A 28-days chronic fluoxetine treatment (Flx, 18 mg/kg/day) reduced corticosterone-induced increase in emotional behavior. A 50% decrease in emotionality score threshold before and after Flx, was used to separate Flx-responding mice (Flx-R, n=18), or Flx non-responder mice (Flx-NR, n=7). Then, Flx-NR mice received 7 sessions of electroconvulsive seizure (ECS, equivalent to ECT in humans) and blood was collected before and after ECS treatment. Chronic ECS normalized the elevated emotionality observed in Flx-NR mice. Then, proteins were extracted from peripheral blood mononuclear cells (PBMCs) and isolated for proteomic analysis using a high-resolution MS Orbitrap. The proteomic analysis revealed a signature of 33 peripheral proteins associated with response to ECS (7 down- and 26 upregulated). These proteins were previously associated with mental disorders and involved in regulating pathways which participate to the depressive disorder etiology. remark for sample name : ECTR=Flx-NR-ES, C=cort/Veh, CFNR= Flx-NR
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:59:30.087.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Céline Henry
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-13 15:12:15	ID requested
1	2022-11-01 02:33:33	announced
⏵ 2	2023-11-14 08:59:30	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: PBMC, depression, blood, antidepressant,treatment resistant depression, electrovonvulsive seizure

Dr Jean-Philippe Guiloux
contact affiliation	Inserm, CESP, Equipe « Moods » Université Paris Saclay - Batiment Henri Moissan 19 avenue des Sciences 91400 Orsay France
contact email	jean-philippe.guilloux@universite-paris-saclay.fr
lab head
Céline Henry
contact affiliation	INRAE
contact email	celine.henry@inrae.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037392
     1. Label: PRIDE project
     2. Name: Peripheral proteomic changes after electroconvulsive seizures (ECS) in a rodent model of non-respons to chronic fluoxetine