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PXD037346-1

PXD037346 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA proteomic analysis shows stimulation of light reactions and inhibition of Calvin cycle in skin chloroplasts of ripe red grape berries
DescriptionThe role of photosynthesis in fruits still challenges scientists. This is especially true in the case of mature grape berries of red varieties lined by an anthocyanin-enriched exocarp (skin) almost impermeable to gases. Although chlorophylls are degraded and replaced by carotenoids in several fruits, available evidence suggests that they may persist in red grapes at maturity. In the present study, chloroplasts were isolated from the skin of red grape berries (cv. Vinhão) to measure chlorophyll levels and the organelle proteome. The results showed that chloroplasts (and chlorophylls) are maintained in ripe berries masked by anthocyanin accumulation and that the proteome of chloroplasts from green and mature berries is distinct. Several proteins of the light reactions significantly accumulated in chloroplasts at the mature stage, including those of light-harvesting complexes of photosystems I (PSI) and II (PSII), redox chain, and ATP synthase, while chloroplasts at the green stage accumulated more proteins involved in the Calvin cycle and the biosynthesis of amino acids, including precursors of secondary metabolism. Taken together, results suggest that although chloroplasts are more involved in biosynthetic reactions in green berries, at the mature stage, they may provide ATP for energy-demanding processes such as anthocyanin biosynthesis or even O2 to feed the respiratory demand of inner tissues.
HostingRepositoryPRIDE
AnnounceDate2022-10-25
AnnouncementXMLSubmission_2022-10-25_09:49:42.202.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAntónioTeixeira
SpeciesList scientific name: Vitis vinifera (Grape); NCBI TaxID: 29760;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-10-12 14:02:07ID requested
12022-10-25 09:49:42announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Grape berry skin, Chloroplasts, Photosynthesis, Vitis vinifera, Proteomics
Contact List
Antónioteixeira
contact affiliationCentre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Braga, Portugal
contact emailantonio.teixeira@bio.uminho.pt
lab head
AntónioTeixeira
contact affiliationCentre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Braga, Portugal
contact emailantonio.teixeira@bio.uminho.pt
dataset submitter
Full Dataset Link List
Dataset FTP location
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