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PXD037345

PXD037345 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe non venomous spider Uloborus sp. (Uloboridae) midgut proteome
DescriptionSpiders have distinct capture prey behaviors selected along Araneae´s evolutive history, but mainly based on the use of venom for prey paralysis. Uloboridae spiders lost the venom glands secondarily in evolution. Due to that they extensively wrap prey with silk to paralyze and begin digestion. During the extra-oral digestion, the digestive fluid very efficiently performs the liquefaction of both the prey and the AcSp2 spidroins from the web fibers. Despite the efficiency of this process, the cocktail of enzymes involved in digestion in Uloboridae spiders is unknown. In this study, we evaluated the protein content in the midgut of Uloborus sp. using enzymatic, proteomic, and phylogenetic analysis approaches. Hydrolases as peptidases (endo and exopeptidases: cysteine, serine and metallopeptidases), carbohydrases (alpha-amylase, chitinase, alpha-mannosidase), and lipases were biochemically assayed; 50 proteins, annotated as enzymes, structural proteins, and toxins, were identified. This is the first characterization of the molecules involved in the digestive process and the midgut protein content of a nonvenomous spider.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:43:34.421.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD037345
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterRodrigo Valladão
SpeciesList scientific name: Uloborus; NCBI TaxID: 94032;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentShimadzu instrument model
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-10-12 14:01:48ID requested
12023-03-11 10:34:27announced
22023-11-14 08:43:36announced2023-11-14: Updated project metadata.
Publication List
Vallad, ã, o R, Neto OBS, de Oliveira Gonzaga M, Pimenta DC, Lopes AR, Digestive enzymes and sphingomyelinase D in spiders without venom (Uloboridae). Sci Rep, 13(1):2661(2023) [pubmed]
Keyword List
submitter keyword: Spider, Digestion, Venom, Enzyme
Contact List
Adriana Rios Lopes
contact affiliationButantan Institute, Biochemistry Laboratory, São Paulo, 05503900, Brazil
contact emailadriana.lopes@butantan.gov.br
lab head
Rodrigo Valladão
contact affiliationUniversidade de São Paulo (USP)
contact emailrodrigovalladao3@gmail.com
dataset submitter
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Dataset FTP location
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