PXD037345 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The non venomous spider Uloborus sp. (Uloboridae) midgut proteome |
Description | Spiders have distinct capture prey behaviors selected along Araneae´s evolutive history, but mainly based on the use of venom for prey paralysis. Uloboridae spiders lost the venom glands secondarily in evolution. Due to that they extensively wrap prey with silk to paralyze and begin digestion. During the extra-oral digestion, the digestive fluid very efficiently performs the liquefaction of both the prey and the AcSp2 spidroins from the web fibers. Despite the efficiency of this process, the cocktail of enzymes involved in digestion in Uloboridae spiders is unknown. In this study, we evaluated the protein content in the midgut of Uloborus sp. using enzymatic, proteomic, and phylogenetic analysis approaches. Hydrolases as peptidases (endo and exopeptidases: cysteine, serine and metallopeptidases), carbohydrases (alpha-amylase, chitinase, alpha-mannosidase), and lipases were biochemically assayed; 50 proteins, annotated as enzymes, structural proteins, and toxins, were identified. This is the first characterization of the molecules involved in the digestive process and the midgut protein content of a nonvenomous spider. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:43:34.421.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD037345 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Rodrigo Valladão |
SpeciesList | scientific name: Uloborus; NCBI TaxID: 94032; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Shimadzu instrument model |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-10-12 14:01:48 | ID requested | |
1 | 2023-03-11 10:34:27 | announced | |
⏵ 2 | 2023-11-14 08:43:36 | announced | 2023-11-14: Updated project metadata. |
Publication List
Vallad, ã, o R, Neto OBS, de Oliveira Gonzaga M, Pimenta DC, Lopes AR, Digestive enzymes and sphingomyelinase D in spiders without venom (Uloboridae). Sci Rep, 13(1):2661(2023) [pubmed] |
Keyword List
submitter keyword: Spider, Digestion, Venom, Enzyme |
Contact List
Adriana Rios Lopes |
contact affiliation | Butantan Institute, Biochemistry Laboratory, São Paulo, 05503900, Brazil |
contact email | adriana.lopes@butantan.gov.br |
lab head | |
Rodrigo Valladão |
contact affiliation | Universidade de São Paulo (USP) |
contact email | rodrigovalladao3@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD037345
- Label: PRIDE project
- Name: The non venomous spider Uloborus sp. (Uloboridae) midgut proteome