Updated project metadata. Spiders have distinct capture prey behaviors selected along Araneae´s evolutive history, but mainly based on the use of venom for prey paralysis. Uloboridae spiders lost the venom glands secondarily in evolution. Due to that they extensively wrap prey with silk to paralyze and begin digestion. During the extra-oral digestion, the digestive fluid very efficiently performs the liquefaction of both the prey and the AcSp2 spidroins from the web fibers. Despite the efficiency of this process, the cocktail of enzymes involved in digestion in Uloboridae spiders is unknown. In this study, we evaluated the protein content in the midgut of Uloborus sp. using enzymatic, proteomic, and phylogenetic analysis approaches. Hydrolases as peptidases (endo and exopeptidases: cysteine, serine and metallopeptidases), carbohydrases (alpha-amylase, chitinase, alpha-mannosidase), and lipases were biochemically assayed; 50 proteins, annotated as enzymes, structural proteins, and toxins, were identified. This is the first characterization of the molecules involved in the digestive process and the midgut protein content of a nonvenomous spider.