PXD037307-1
PXD037307 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | GPI-Anchored Ligand-BioID2-Tagging System Identifies proteins Mediating Zika Virus Entry |
Description | Identification of host factors facilitating pathogen entry is critical for preventing infectious diseases. Here, we report a tagging system consisting of a viral receptor-binding protein (RBP) linked to BioID2, which is expressed on the cell surface via a GPI anchor. Using VSV or Zika virus (ZIKV) RBP, the system (BioID2- RBP(V)-GPI; BioID2-RBP(Z)-GPI) faithfully identifies LDLR and AXL, the receptors of VSV and ZIKV, respectively. Being GPI-anchored is essential for the probe to function properly. Furthermore, BioID2-RBP(Z)-GPI expressed in human neuronal progenitor cells identifies galectin-1 on cell surface pivotal for ZIKV entry. This conclusion is further supported by antibody blocking and galectin-1 silencing in A549 and mouse neural cells. Importantly, Lgas1-/- mice are significantly more resistant to ZIKV infection than Lgas1+/+ littermates are, having significantly lower virus titers and fewer pathologies in various organs. This tagging system may have broad applications for identifying protein-protein interactions on the cell surface |
HostingRepository | iProX |
AnnounceDate | 2022-10-12 |
AnnouncementXML | Submission_2022-10-12_01:16:10.796.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Shanshan Gao |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Bruker Daltonics amaZon series |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2022-10-12 01:14:59 | ID requested | |
⏵ 1 | 2022-10-12 01:16:11 | announced | |
2 | 2023-12-06 19:11:30 | announced | 2023-12-07: Update information. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Proximity labeling, biotin ligase, GPI anchor, lipid raft, Zika virus,Galectin-1 |
Contact List
Chaoyang Li | |
---|---|
contact affiliation | Affiliated Cancer Hospital and Institute of Guangzhou Medical University, State Key Laboratory of Respiratory Disease, Key Laboratory for Cell Homeostasis and Cancer Research of Guangdong High Education Institute |
contact email | chaoyangli@gzhmu.edu.cn |
lab head | |
Shanshan Gao | |
contact affiliation | Wuhan Institute of Virology, Chinese Academy of Sciences |
contact email | gaoshanshan202207@163.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |