PXD037307
PXD037307 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | GPI-Anchored Ligand-BioID2-Tagging System Identifies proteins Mediating Zika Virus Entry |
Description | Identification of host factors facilitating pathogen entry is critical for preventing infectious diseases. Here, we report a tagging system consisting of a viral receptor-binding protein (RBP) linked to BioID2, which is expressed on the cell surface via a GPI anchor. Using VSV or Zika virus (ZIKV) RBP, the system (BioID2- RBP(V)-GPI; BioID2-RBP(Z)-GPI) faithfully identifies LDLR and AXL, the receptors of VSV and ZIKV, respectively. Being GPI-anchored is essential for the probe to function properly. Furthermore, BioID2-RBP(Z)-GPI expressed in human neuronal progenitor cells identifies galectin-1 on cell surface pivotal for ZIKV entry. This conclusion is further supported by antibody blocking and galectin-1 silencing in A549 and mouse neural cells. Importantly, Lgas1-/- mice are significantly more resistant to ZIKV infection than Lgas1+/+ littermates are, having significantly lower virus titers and fewer pathologies in various organs. This tagging system may have broad applications for identifying protein-protein interactions on the cell surface |
HostingRepository | iProX |
AnnounceDate | 2022-10-12 |
AnnouncementXML | Submission_2023-12-06_19:11:30.098.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Shanshan Gao |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Bruker Daltonics amaZon series |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2022-10-12 01:14:59 | ID requested | |
1 | 2022-10-12 01:16:11 | announced | |
⏵ 2 | 2023-12-06 19:11:30 | announced | 2023-12-07: Update information. |
Publication List
Gao SS, Shi R, Sun J, Tang Y, Zheng Z, Li JF, Li H, Zhang J, Leng Q, Xu J, Chen X, Zhao J, Sy MS, Feng L, Li C, GPI-anchored ligand-BioID2-tagging system identifies Galectin-1 mediating Zika virus entry. iScience, 25(12):105481(2022) [pubmed] |
Keyword List
submitter keyword: Proximity labeling, biotin ligase, GPI anchor, lipid raft, Zika virus,Galectin-1 |
Contact List
Chaoyang Li | |
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contact affiliation | Affiliated Cancer Hospital and Institute of Guangzhou Medical University, State Key Laboratory of Respiratory Disease, Key Laboratory for Cell Homeostasis and Cancer Research of Guangdong High Education Institute |
contact email | chaoyangli@gzhmu.edu.cn |
lab head | |
Shanshan Gao | |
contact affiliation | Wuhan Institute of Virology, Chinese Academy of Sciences |
contact email | gaoshanshan202207@163.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |