PXD037284-1

PXD037284 is an original dataset announced via ProteomeXchange.

Title	Proteome reveal abnormal spermatozoa in precocious Chinese mitten crab, Eriocheir sinensis
Description	In juvenile Chinese mitten crabs, Eriocheir sinensis, the occurrence of sexual gland maturation in the first year is known as precocity and significantly affects culture productivity and profitability. The miniaturization of individuals is a key characteristic of precocious crabs. Most related studies focus on the underlying causes, nutritional quality, biochemical components and differentially expressed mRNAs of precocious crab individuals and tissues. The reproductive performance of precocious crabs is evaluated based on offspring quality. However, the status of precocious parental gametes (especially spermatozoa) directly related to the offspring has not been reported. To clarify the differences in spermatozoa between precocious and normal mature crabs, spermatozoal proteome data was obtained via high-throughput sequencing technology. The results revealed a total of 856 differentially expressed genes and 150 differentially expressed proteins in spermatozoa. Functional analysis showed that these genes and proteins identified in the spermatozoa of precocious E. sinensis were significantly enriched in the categories of substance and energy metabolism. And more, the miniaturization of spermatogenic cells was also identified in precocious crabs. This indicates that metabolic disorders might be the main reason for the miniaturization of spermatogenic cells in precocious E. sinensis males. The molecular defects in precocious sperm were closely related to the imbalances in essential substance and energy metabolism. These findings provide foundational information and insights into hidden biological irregularities associated with precocious phenotypes and will contribute to the further study of precocious E. sinensis males and even other crustaceans.
HostingRepository	iProX
AnnounceDate	2022-10-11
AnnouncementXML	Submission_2022-10-10_21:46:00.920.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Huan Liu
SpeciesList	scientific name: Eriocheir sinensis; NCBI TaxID: 95602;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-10 21:45:47	ID requested
⏵ 1	2022-10-10 21:46:01	announced

Dataset with its publication pending

submitter keyword: Eriocheir sinensis, Precocious puberty, Sperm proteome, Abnormal sperm metabolism

Xianjiang Kang
contact affiliation	Hebei University
contact email	xjkang@hbu.edu.cn
lab head
Huan Liu
contact affiliation	Hebei University
contact email	15831199596@163.com
dataset submitter

iProX dataset URI