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PXD037250-2

PXD037250 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic and in silico analyses of the influence of dextran synthesis on the adaptation of Leuconostoc lactis AV1n to temperature change
DescriptionLeuconostoc lactis is found in vegetables, fruits, and meat and is used by the food industry in the preparation of dairy products, wines and sugars. We have previously demonstrated, that the dextransucrase (DsrLL) of Lc. lactis AV1n produces a high molecular weight dextran from sucrose, indicating its potential use as a dextran forming starter culture. We have also shown that this bacterium was able to produce 10-fold higher levels of dextran at 20 °C than at 37 °C, at the former temperature accompanied by an increase of dsrLL gene expression. However, the general physiological response of Lc. lactis AV1n to cold temperature in the presence of sucrose, leading to an increased production of dextran has not been yet investigated. Therefore, we have here used a quantitative proteomics approach to investigate the cold temperature-induced changes in the proteomic profile of this strain in comparison to its proteomic response at 37 °C. In total, 337 proteins were found to be differentially expressed at the applied significance criteria (FDR-adjusted p-value ≤ 0.05 and with a fold change ≥ 1.5 or ≤ 0.67) with 204 proteins overexpressed, among which 13% were involved in protein as well as cell wall, and envelope component biosynthesis including DsrLL as RNA chaperones. Proteins implicated in cold stress were expressed at high level at 20 °C and possibly play a role in the upregulation of DsrLL allowing efficient synthesis of the protein essential for its adaptation to cold. Post-transcriptional regulation of DsrLL expression also seems to take place through the interplay of exonucleases and endonucleases overexpressed at 20 °C, that would influence the half-life of the dsrLL transcript. Furthermore, the mechanism of cold resistance of Lc. lactis AV1n seems to be also based on energy saving through a decrease in growth rate mediated by a decrease in carbohydrate metabolism and its orientation towards the production pathways for storage molecules. Thus, this better understanding of the responses to low temperature and mechanisms for environmental adaptation of Lc. lactis could be exploited for industrial use of strains belonging to this species.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:59:04.508.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterVivian de los Rios
SpeciesList scientific name: Leuconostoc lactis; NCBI TaxID: 1246;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-10-10 07:43:00ID requested
12022-12-27 10:46:01announced
22023-11-14 08:59:05announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP), Food and Nutrition (B/D-HPP), Human Proteome Project
submitter keyword: Leuconostoc, lactic acid bacteria, proteomic, dextran, exopolysaccharides
Contact List
Paloma López
contact affiliationCenter for Biological Research (CIB-CSIC)
contact emailplg@cib.csic.es
lab head
Vivian de los Rios
contact affiliationCIB.CSIC
contact emailvrios@cib.csic.es
dataset submitter
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