<<< Full experiment listing

PXD037153-1

PXD037153 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleNonionic surfactants can modify the thermal stability of globular and membrane proteins interfering with the thermal proteome profiling principles to identify protein targets
DescriptionThe membrane proteins are essential targets to understand cellular function. The unbiased identification of membrane protein targets is still the bottleneck for a system- level understanding of cellular response to stimuli or perturbations. It has been suggested to enrich the soluble proteome with membrane proteins by introducing nonionic surfactants in the solubilization solution. This strategy was aiming to simultaneous identify the globular and membrane protein targets by thermal proteome profiling principles. However, the thermal shift assay would surpass the cloud point temperature from the nonionic surfactants most frequently utilized for membrane protein solubilization. It is expected that around the cloud point temperature, the surfactant micelles would suffer structural modifications altering the proteome solubility. Here, we show that the presence of nonionic surfactants can alter protein thermal stability from a mixed globular, and membrane proteome. In the presence of surfactant micelles, the changes in protein solubility analyzed after the thermal shift assay are affected by the thermal dependent modification of the micelles size, and their interaction with proteins. We demonstrate that the introduction of nonionic surfactants for the solubilization of membrane proteins is not compatible with the principles of target identification by thermal proteome profiling methodologies. Our results lead to explore thermal-independent strategies for membrane protein solubilization to assure confident membrane protein target identification. The proteome-wide thermal shift methods have already shown their capability to elucidate mechanism of actions from pharma, biomedicine, analytical chemistry, or toxicology and finding strategies, free from surfactants, to identify membrane protein targets would be the next challenge.
HostingRepositoryPRIDE
AnnounceDate2023-03-20
AnnouncementXMLSubmission_2023-03-20_08:00:22.928.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterVeronicaLizano-Fallas
SpeciesList scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-10-03 11:20:02ID requested
12023-03-20 08:00:23announced
22023-11-14 08:59:12announced2023-11-14: Updated project metadata.
Publication List
Keyword List
submitter keyword: rat liver tissue, Igepal, micelles, nonionic surfactants,Thermal proteome profiling, Nonidet-P40 substitute
Contact List
SusanaCristobal
contact affiliationDepartment of Biomedical and Clinical Sciences, Cell Biology, Faculty of Medicine, Linköping University, Linköping, Sweden
contact emailsusana.cristobal@liu.se
lab head
VeronicaLizano-Fallas
contact affiliationLinköping University
contact emailveronica.lizano@liu.se
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD037153
PRIDE project URI
Repository Record List
[ + ]