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PXD037079-1

PXD037079 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTMT-Based Quantitative Proteomics Reveals the Targets of Andrographolide on LPS-induced Liver Injury
DescriptionGram-negative bacterial infections can cause varying degrees of liver injury in chickens. Although andrographolide has been shown to have a protective effect on the liver, its underlying mechanism of action and effects on liver proteins are not known. The study objectives were to analyze the actions of andrographolide on lipopolysaccharide (LPS)-induced chicken leghorn male hepatoma (LMH) cell injury, identify the different proteins in different groups using TMT proteomics, and explore the pharmacological effects and potential targets of andrographolide in LPS-induced liver injury. It was found that andrographolide reduced alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in the cell supernatant and alleviated LPS-induced injury in LMH cells. Proteomic analysis identified 50 and 166 differentially expressed proteins in the LPS vs NC group and LPS-Andro vs LPS group, respectively. Andrographolide may be involved in steroid metabolic processes, negative regulation of MAPK cascade, oxidative stress, and other processes to protect against LPS-induced liver injury. HMGCS1, HMGCR, FDPS, PBK, CAV1, PRDX1, PRDX4, and PRDX6, which were identified by differential proteomics, may be the targets of andrographolide. Our study may provide new theoretical support for andrographolide protection against liver injury.
HostingRepositoryiProX
AnnounceDate2022-09-29
AnnouncementXMLSubmission_2022-09-29_21:00:02.464.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterShihao Ge
SpeciesList scientific name: Gallus gallus; NCBI TaxID: 9031;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-09-29 20:59:36ID requested
12022-09-29 21:00:02announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: proteomics, andrographolide, LPS, liver injury
Contact List
Quanhai Pang
contact affiliationShanxi Agricultural University
contact emailpangquanhai@163.com
lab head
Shihao Ge
contact affiliationShanxi Agricultural University&Heze University
contact email53642201@qq.com
dataset submitter
Full Dataset Link List
iProX dataset URI