PXD036985 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Preliminary cell culture models for subclinical mastitis in sheep |
Description | Ovine mastitis is defined as the inflammation of the sheep udder, most commonly caused in response to intramammary infections. Based on the occurrence of clinical signs, mastitis is characterized as either clinical or subclinical (SCM). The impact of ovine SCM on the overall sustainability of dairy sheep farms has been documented underpinning the significance of efficient diagnosis. Although SCM can be detected in cows, the performance and the validity of the methods used do not transfer in dairy sheep. This fact challenges the development of evidence-based ovine udder health management protocols and renders the detection and control of ovine mastitis rather problematic. Currently, cell culture-based models are being successfully used in biomedical studies and have also been effectively used in the case of bovine mastitis. The objective of the present study was to culture ovine primary mammary cells for the development of 2D and 3D cell culture-based models for the study of ovine SCM. Cells were infected by mastitis-inducing pathogens mimicking the pathogenesis of SCM as derived by natural intramammary infections. The secreted proteins were subjected to mass-spectrometry resulting in the identification of 79 distinct proteins. Among those, several had already been identified in healthy or mastitic milk, while others were detected for the first time in the ovine mammary secretome. The development of cell-based models for the early detection and the overall study of SCM has the potential to be applicable and beneficial for the udder health management in dairy sheep. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_07:32:36.575.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Georgios Theodorou |
SpeciesList | scientific name: Ovis aries; NCBI TaxID: 9940; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-09-25 14:47:19 | ID requested | |
1 | 2022-11-03 02:12:24 | announced | |
⏵ 2 | 2023-11-14 07:32:36 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: cell culture models,Mastitis, secreted proteins, ovine, in vitro infection |
Contact List
Georgios Theodorou |
contact affiliation | Laboratory of Animal Breeding & Husbandry, Department of Animal Science, Agricultural University of Athens, Athens, Greece |
contact email | gtheod@aua.gr |
lab head | |
Georgios Theodorou |
contact affiliation | Agricultural University of Athens, Department of Animal Science, Laboratory of Animal Breeding & Husbandry |
contact email | gtheod@aua.gr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD036985
- Label: PRIDE project
- Name: Preliminary cell culture models for subclinical mastitis in sheep