PXD036985

PXD036985 is an original dataset announced via ProteomeXchange.

Title	Preliminary cell culture models for subclinical mastitis in sheep
Description	Ovine mastitis is defined as the inflammation of the sheep udder, most commonly caused in response to intramammary infections. Based on the occurrence of clinical signs, mastitis is characterized as either clinical or subclinical (SCM). The impact of ovine SCM on the overall sustainability of dairy sheep farms has been documented underpinning the significance of efficient diagnosis. Although SCM can be detected in cows, the performance and the validity of the methods used do not transfer in dairy sheep. This fact challenges the development of evidence-based ovine udder health management protocols and renders the detection and control of ovine mastitis rather problematic. Currently, cell culture-based models are being successfully used in biomedical studies and have also been effectively used in the case of bovine mastitis. The objective of the present study was to culture ovine primary mammary cells for the development of 2D and 3D cell culture-based models for the study of ovine SCM. Cells were infected by mastitis-inducing pathogens mimicking the pathogenesis of SCM as derived by natural intramammary infections. The secreted proteins were subjected to mass-spectrometry resulting in the identification of 79 distinct proteins. Among those, several had already been identified in healthy or mastitic milk, while others were detected for the first time in the ovine mammary secretome. The development of cell-based models for the early detection and the overall study of SCM has the potential to be applicable and beneficial for the udder health management in dairy sheep.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:32:36.575.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Georgios Theodorou
SpeciesList	scientific name: Ovis aries; NCBI TaxID: 9940;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-25 14:47:19	ID requested
1	2022-11-03 02:12:24	announced
⏵ 2	2023-11-14 07:32:36	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: cell culture models,Mastitis, secreted proteins, ovine, in vitro infection

Georgios Theodorou
contact affiliation	Laboratory of Animal Breeding & Husbandry, Department of Animal Science, Agricultural University of Athens, Athens, Greece
contact email	gtheod@aua.gr
lab head
Georgios Theodorou
contact affiliation	Agricultural University of Athens, Department of Animal Science, Laboratory of Animal Breeding & Husbandry
contact email	gtheod@aua.gr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/11/PXD036985

PRIDE project URI

• PRIDE
  1. PXD036985
     1. Label: PRIDE project
     2. Name: Preliminary cell culture models for subclinical mastitis in sheep