PXD036983 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | : Proximity proteomics identifies endothelial cell septins and PAK2 as decisive regulators of actomyosin mediated expulsion of von Willebrand factor. |
Description | A Rab27a targeted APEX2 construct was transfected into primary human umbilical endothelial cells (HUVEC) and the proximal proteins surrounding endothelial storage organelles (Weibel Palade bodies) identified by proximity proteomics in unstimulated and stimulated conditions. The procedure was carried out in the presence and absence of two different stimuli (10 minutes-phorbol myristate acetate or a combination of Histamine, adrenalin and 3-isobutyl-1-methylxanthine) and the data presented represents 4 independent experiments each with 2 technical replicates. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:23:44.811.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD036983 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Vinothini Rajeeve |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue; monohydroxylated residue; iodoacetic acid derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-09-25 14:36:53 | ID requested | |
1 | 2023-01-11 03:31:10 | announced | |
⏵ 2 | 2023-11-14 08:23:47 | announced | 2023-11-14: Updated project metadata. |
Publication List
10.6019/PXD036983; |
El-Mansi S, Robinson CL, Kostelnik KB, McCormack JJ, Mitchell TP, Lobato-M, รก, rquez D, Rajeeve V, Cutillas P, Cutler DF, Mostowy S, Nightingale TD, Proximity proteomics identifies septins and PAK2 as decisive regulators of actomyosin-mediated expulsion of von Willebrand factor. Blood, 141(8):930-944(2023) [pubmed] |
Keyword List
submitter keyword: Rab27a, PAK2, VWF secretion, proteomics, proximity proteomics, Septin, endothelial cells,APEX2, haemostasis, Weibel palade bodies, LC-MS/MS |
Contact List
Professor Pedro R Cutillas |
contact affiliation | Centre for Genomics and Computational Biology Barts Cancer Institute Queen Mary, University of London John Vane Science Centre, Charterhouse Square, London EC1M 6BQ |
contact email | p.cutillas@qmul.ac.uk |
lab head | |
Vinothini Rajeeve |
contact affiliation | Centre for Haemato Oncology |
contact email | v.rajeeve@qmul.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD036983
- Label: PRIDE project
- Name: : Proximity proteomics identifies endothelial cell septins and PAK2 as decisive regulators of actomyosin mediated expulsion of von Willebrand factor.