PXD036983

PXD036983 is an original dataset announced via ProteomeXchange.

Title	: Proximity proteomics identifies endothelial cell septins and PAK2 as decisive regulators of actomyosin mediated expulsion of von Willebrand factor.
Description	A Rab27a targeted APEX2 construct was transfected into primary human umbilical endothelial cells (HUVEC) and the proximal proteins surrounding endothelial storage organelles (Weibel Palade bodies) identified by proximity proteomics in unstimulated and stimulated conditions. The procedure was carried out in the presence and absence of two different stimuli (10 minutes-phorbol myristate acetate or a combination of Histamine, adrenalin and 3-isobutyl-1-methylxanthine) and the data presented represents 4 independent experiments each with 2 technical replicates.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:23:44.811.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD036983
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Vinothini Rajeeve
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetic acid derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-25 14:36:53	ID requested
1	2023-01-11 03:31:10	announced
⏵ 2	2023-11-14 08:23:47	announced	2023-11-14: Updated project metadata.

10.6019/PXD036983;

El-Mansi S, Robinson CL, Kostelnik KB, McCormack JJ, Mitchell TP, Lobato-M, á, rquez D, Rajeeve V, Cutillas P, Cutler DF, Mostowy S, Nightingale TD, Proximity proteomics identifies septins and PAK2 as decisive regulators of actomyosin-mediated expulsion of von Willebrand factor. Blood, 141(8):930-944(2023) [pubmed]

submitter keyword: Rab27a, PAK2, VWF secretion, proteomics, proximity proteomics, Septin, endothelial cells,APEX2, haemostasis, Weibel palade bodies, LC-MS/MS

Professor Pedro R Cutillas
contact affiliation	Centre for Genomics and Computational Biology Barts Cancer Institute Queen Mary, University of London John Vane Science Centre, Charterhouse Square, London EC1M 6BQ
contact email	p.cutillas@qmul.ac.uk
lab head
Vinothini Rajeeve
contact affiliation	Centre for Haemato Oncology
contact email	v.rajeeve@qmul.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/01/PXD036983

PRIDE project URI

• PRIDE
  1. PXD036983
     1. Label: PRIDE project
     2. Name: : Proximity proteomics identifies endothelial cell septins and PAK2 as decisive regulators of actomyosin mediated expulsion of von Willebrand factor.