PXD036844

PXD036844 is an original dataset announced via ProteomeXchange.

Title	Proteomic landscape of plasma and plasma-derived sEVs and lEVs in uveitis and posterior scleritis
Description	Uveitis and posterior scleritis are sight-threatening diseases with undefined pathogenesis, accurate diagnosis remains challenging. Here, two plasma-derived extracellular vesicle (EV) subpopulations, small and large EVs, were obtained from patients with uveitis and posterior scleritis and subjected to proteomics analysis alongside plasma. We identify 3,658 proteins, with 3,097, 3,312, and 2,820 quantifiable proteins for sEVs, lEVs, and plasma, respectively. The proteomes of the two EV subgroups differed significantly and were more highly correlated with disease development than that of plasma. Comprehensive bioinformatics analysis of the EVs and plasma highlighted a potential pathogenic mechanism for these diseases. Four potential biomarker panels for four diseases were identified and validated. We found a negative correlation between plasma endothelin-converting enzyme 1 levels and mean retinal thickness. This study provides a proteomic landscape of the molecular changes in plasma and EVs involved in four diseases, offers insights into their potential pathogenesis, and identifies valuable biomarker candidates.
HostingRepository	iProX
AnnounceDate	2022-09-19
AnnouncementXML	Submission_2023-06-25_20:04:04.835.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lingzi Wu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-19 01:29:05	ID requested
1	2022-09-19 01:29:37	announced
⏵ 2	2023-06-25 20:04:10	announced	2023-06-26: Update publication information.

Wu L, Zhou L, An J, Shao X, Zhang H, Wang C, Zhao G, Chen S, Cui X, Zhang X, Yang F, Li X, Zhang X, Comprehensive profiling of extracellular vesicles in uveitis and scleritis enables biomarker discovery and mechanism exploration. J Transl Med, 21(1):388(2023) [pubmed]

submitter keyword: uveitis, posterior scleritis, plasma, small extracellular vesicles, large extracellular vesicles, proteomic profiles, biomarkers

Xiaomin Zhang
contact affiliation	Tianjin Medical University Eye Hospital
contact email	xzhang08@tmu.edu.cn
lab head
Lingzi Wu
contact affiliation	Tianjin Medical University Eye Hospital
contact email	wlz_5206@tmu.edu.cn
dataset submitter

iProX dataset URI