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PXD036809-1

PXD036809 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe histone chaperone NASP maintains H3-H4 reservoirs in the early Drosophila embryo
DescriptionHistones are essential for chromatin packaging and histone supply must be tightly regulated as excess histones are toxic. To drive the rapid cell cycles of the early embryo, however, excess histones are maternally deposited. Therefore, soluble histones must be buffered by histone chaperones but the chaperone necessary to stabilize soluble H3-H4 pools in the Drosophila embryo has yet to be identified. Here, we show that CG8223, the Drosophila ortholog of NASP, is a H3-H4-specific chaperone in the early embryo. NASP specifically binds to H3-H4 in the early embryo. We demonstrate that, while NASP is non-essential in Drosophila, NASP is maternal effect lethal gene. Embryos laid by NASP mutant mothers have a reduce rate of hatching and show defects in early embryogenesis. Critically, soluble H3-H4 pools are degraded in embryos laid by NASP mutant mothers. Our work identifies NASP as the critical H3-H4 histone chaperone in the Drosophila embryo.
HostingRepositoryPRIDE
AnnounceDate2023-02-23
AnnouncementXMLSubmission_2023-02-23_07:19:57.558.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJonathanDavies
SpeciesList scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-09-17 13:49:39ID requested
12023-02-23 07:19:57announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: histone,NASP, H3-H4, histone chaperone, IP-MS
Contact List
Jared T.Nordman
contact affiliationDepartment of Biological Sciences, Vanderbilt University
contact emailjared.nordman@vanderbilt.edu
lab head
JonathanDavies
contact affiliationVanderbilt University
contact emailjonathan.p.davies@vanderbilt.edu
dataset submitter
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Dataset FTP location
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