PXD036774 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | LAMP3-associated extracellular vesicles induce apoptosis in Sjögren’s disease |
Description | Sjögren’s disease (SjD) is an autoimmune disease that affects exocrine tissues and is characterized by increased apoptosis and decreased expression of membrane proteins involved in secretory function, such as Na-K-Cl cotransporter-1 (NKCC1), in salivary and lacrimal glands. Although the pathogenic mechanism triggering SjD is not well understood, overexpression of lysosome-associated membrane protein 3 (LAMP3) is associated with the disease in a subset of SjD patients and the development of SjD-like phenotype in mice. In this study, histological analysis of minor salivary glands of SjD patients suggested that LAMP3-containing material is being ejected from cells. Follow-on in vitro experiments with cells exposed to larger extracellular vesicles (LEVs) derived from LAMP3-overexpressing cells showed increased apoptosis and decreased NKCC1 expression. Proteomics identified LAMP3 as a major component of LEVs derived from LAMP3-overexpressing cells. Live-cell imaging visualized release and uptake of LAMP3-containing LEVs from LAMP3-overexpressing cells to naïve cells. Furthermore, experiments with recombinant LAMP3 protein alone or complexed with Xfect protein transfection reagent demonstrated that internalization of LAMP3 was required for apoptosis in a caspase-dependent pathway. Taken together, we identified a new role for extracellular LAMP3 in cell-to-cell communication via LEVs, which provides further support for targeting LAMP3 as a therapeutic approach in SjD. |
HostingRepository | PRIDE |
AnnounceDate | 2023-03-10 |
AnnouncementXML | Submission_2023-03-10_12:17:30.146.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | YanWang |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-09-17 02:27:45 | ID requested | |
⏵ 1 | 2023-03-10 12:17:31 | announced | |
2 | 2023-11-14 08:33:40 | announced | 2023-11-14: Updated project metadata. |
Publication List
Tanaka T, Nakamura H, Tran DT, Warner BM, Wang Y, Atsumi T, Noguchi M, Chiorini JA, gren's disease. Sci Rep, 13(1):2595(2023) [pubmed] |
Keyword List
submitter keyword: cell-to-cell communication, large extracellular vesicle,Sjögren’s syndrome, apoptosis, lysosome-associated membrane protein 3 |
Contact List
YanWang |
contact affiliation | NIH/NIDCR |
contact email | wangyan2@nih.gov |
lab head | |
YanWang |
contact affiliation | NIH/NIDCR |
contact email | wangyan2@nih.gov |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD036774
- Label: PRIDE project
- Name: LAMP3-associated extracellular vesicles induce apoptosis in Sjögren’s disease