PXD036774

PXD036774 is an original dataset announced via ProteomeXchange.

Title	LAMP3-associated extracellular vesicles induce apoptosis in Sjögren’s disease
Description	Sjögren’s disease (SjD) is an autoimmune disease that affects exocrine tissues and is characterized by increased apoptosis and decreased expression of membrane proteins involved in secretory function, such as Na-K-Cl cotransporter-1 (NKCC1), in salivary and lacrimal glands. Although the pathogenic mechanism triggering SjD is not well understood, overexpression of lysosome-associated membrane protein 3 (LAMP3) is associated with the disease in a subset of SjD patients and the development of SjD-like phenotype in mice. In this study, histological analysis of minor salivary glands of SjD patients suggested that LAMP3-containing material is being ejected from cells. Follow-on in vitro experiments with cells exposed to larger extracellular vesicles (LEVs) derived from LAMP3-overexpressing cells showed increased apoptosis and decreased NKCC1 expression. Proteomics identified LAMP3 as a major component of LEVs derived from LAMP3-overexpressing cells. Live-cell imaging visualized release and uptake of LAMP3-containing LEVs from LAMP3-overexpressing cells to naïve cells. Furthermore, experiments with recombinant LAMP3 protein alone or complexed with Xfect protein transfection reagent demonstrated that internalization of LAMP3 was required for apoptosis in a caspase-dependent pathway. Taken together, we identified a new role for extracellular LAMP3 in cell-to-cell communication via LEVs, which provides further support for targeting LAMP3 as a therapeutic approach in SjD.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:33:37.907.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yan Wang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-17 02:27:45	ID requested
1	2023-03-10 12:17:31	announced
⏵ 2	2023-11-14 08:33:40	announced	2023-11-14: Updated project metadata.

Tanaka T, Nakamura H, Tran DT, Warner BM, Wang Y, Atsumi T, Noguchi M, Chiorini JA, gren's disease. Sci Rep, 13(1):2595(2023) [pubmed]

submitter keyword: cell-to-cell communication, large extracellular vesicle,Sjögren’s syndrome, apoptosis, lysosome-associated membrane protein 3

Yan Wang
contact affiliation	NIH/NIDCR
contact email	wangyan2@nih.gov
lab head
Yan Wang
contact affiliation	NIH/NIDCR
contact email	wangyan2@nih.gov
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD036774
     1. Label: PRIDE project
     2. Name: LAMP3-associated extracellular vesicles induce apoptosis in Sjögren’s disease