PXD036751 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic analysis and ligand-binding studies of the odorant-binding protein OBP1 in the elephant's trunk |
Description | Chemical communication in elephants has been studied in detail both at the chemical and at the behavioural levels. Several pheromones have been identified, and their specific effects on the sexual behaviour of both the African and the Asian elephants have been accurately documented. This work is focused on the characterization of OBP1 through ligand-binding studies and analysis of post-translational modifications (PTMs). First, we have performed a proteomic analysis of a crude extract of the trunk wash obtained from the African elephant, and have found OBP1 as the main component, together with minor amounts of a OBP1 isoform and the von Ebner's gland (VEG) protein. Proteomic analysis of OBP1 revealed the occurrence of a variable degree of O-glycosylation, phosphorylation and acetylation in this protein. To specifically assay OBP1 spectra of binding, we have then expressed the African elephant protein in Pichia pastoris, which showed PTMs very similar to the natural counterpart. At functional level, we have found that recombinant OBP1 from the African elephant is able to bind the sex pheromone (Z)-7-dodecenyl acetate with strong affinity and some structurally related esters with lower strength. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:43:14.721.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | CHIARA D'AMBROSIO |
SpeciesList | scientific name: Loxodonta africana (African elephant); NCBI TaxID: 9785; |
ModificationList | phosphorylated residue; acetylated residue; monohydroxylated residue; complex glycosylation; deamidated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-09-14 14:34:32 | ID requested | |
1 | 2023-03-11 10:59:17 | announced | |
⏵ 2 | 2023-11-14 08:43:23 | announced | 2023-11-14: Updated project metadata. |
Publication List
Zaremska V, Renzone G, Arena S, Ciaravolo V, Buberl A, Balfanz F, Scaloni A, Knoll W, Pelosi P, An odorant-binding protein in the elephant's trunk is finely tuned to sex pheromone (Z)-7-dodecenyl acetate. Sci Rep, 12(1):19982(2022) [pubmed] |
Keyword List
submitter keyword: odorant-binding protein |
elephants |
pheromones |
post-translational modifications |
Contact List
Andrea Scaloni |
contact affiliation | Proteomics, Metabolomics & Mass Spectrometry Laboratory, Institute for the Animal Production System in the Mediterranean Environment (ISPAAM), National Research Council, Piazzale Enrico Fermi 1, 80055 Portici (NA), Italy |
contact email | andrea.scaloni@cnr.it |
lab head | |
CHIARA D'AMBROSIO |
contact affiliation | ISPAAM CNR |
contact email | chiara.dambrosio@cnr.it |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD036751
- Label: PRIDE project
- Name: Proteomic analysis and ligand-binding studies of the odorant-binding protein OBP1 in the elephant's trunk