PXD036739 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The Arabidopsis E3 ubiquitin ligase PUB4 regulates BIK1 and is targeted by a bacterial type-III effector |
Description | Plant immunity is tightly controlled by a complex and dynamic regulatory network, which ensures optimal activation upon detection of potential pathogens. Accordingly, each component of this network is a potential target for manipulation by pathogens. Here, we report that RipAC, a type III-secreted effector from the bacterial pathogen Ralstonia solanacearum, targets the plant E3 ubiquitin ligase PUB4 to inhibit pattern-triggered immunity (PTI). PUB4 plays a positive role in PTI by regulating the homeostasis of the central immune kinase BIK1. Before PAMP perception, PUB4 promotes the degradation of non-activated BIK1, while, after PAMP perception, PUB4 contributes to the accumulation of activated BIK1. RipAC leads to BIK1 degradation, which correlates with its PTI-inhibitory activity. RipAC causes a reduction in pathogen-associated molecular pattern (PAMP)-induced PUB4 accumulation and phosphorylation. Our results shed light on the role played by PUB4 in immune regulation, and illustrate an indirect targeting of the immune signalling hub BIK1 by a bacterial effector |
HostingRepository | PRIDE |
AnnounceDate | 2022-10-12 |
AnnouncementXML | Submission_2022-10-12_09:53:35.142.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD036739 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | FRankMenke |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | phosphorylated residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-09-14 14:17:45 | ID requested | |
⏵ 1 | 2022-10-12 09:53:35 | announced | |
Publication List
Keyword List
submitter keyword: Arabidopsis, protein protein interaction,Plant immunity, effector, phosphorylation |
Contact List
Frank L.H.Menke |
contact affiliation | The Sainsbury Laboratory |
contact email | frank.menke@tsl.ac.uk |
lab head | |
FRankMenke |
contact affiliation | the Sainsbury laboratory |
contact email | Frank.Menke@tsl.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD036739 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD036739
- Label: PRIDE project
- Name: The Arabidopsis E3 ubiquitin ligase PUB4 regulates BIK1 and is targeted by a bacterial type-III effector