PXD036739

PXD036739 is an original dataset announced via ProteomeXchange.

Title	The Arabidopsis E3 ubiquitin ligase PUB4 regulates BIK1 and is targeted by a bacterial type-III effector
Description	Plant immunity is tightly controlled by a complex and dynamic regulatory network, which ensures optimal activation upon detection of potential pathogens. Accordingly, each component of this network is a potential target for manipulation by pathogens. Here, we report that RipAC, a type III-secreted effector from the bacterial pathogen Ralstonia solanacearum, targets the plant E3 ubiquitin ligase PUB4 to inhibit pattern-triggered immunity (PTI). PUB4 plays a positive role in PTI by regulating the homeostasis of the central immune kinase BIK1. Before PAMP perception, PUB4 promotes the degradation of non-activated BIK1, while, after PAMP perception, PUB4 contributes to the accumulation of activated BIK1. RipAC leads to BIK1 degradation, which correlates with its PTI-inhibitory activity. RipAC causes a reduction in pathogen-associated molecular pattern (PAMP)-induced PUB4 accumulation and phosphorylation. Our results shed light on the role played by PUB4 in immune regulation, and illustrate an indirect targeting of the immune signalling hub BIK1 by a bacterial effector
HostingRepository	PRIDE
AnnounceDate	2022-10-12
AnnouncementXML	Submission_2022-10-12_09:53:35.142.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD036739
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	FRankMenke
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-14 14:17:45	ID requested
⏵ 1	2022-10-12 09:53:35	announced

10.6019/PXD036739;

submitter keyword: Arabidopsis, protein protein interaction,Plant immunity, effector, phosphorylation

Frank L.H.Menke
contact affiliation	The Sainsbury Laboratory
contact email	frank.menke@tsl.ac.uk
lab head
FRankMenke
contact affiliation	the Sainsbury laboratory
contact email	Frank.Menke@tsl.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD036739
     1. Label: PRIDE project
     2. Name: The Arabidopsis E3 ubiquitin ligase PUB4 regulates BIK1 and is targeted by a bacterial type-III effector