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PXD036659-1

PXD036659 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleOn the relationship between metabolic capacities and in vivo viscoelastic properties of the liver
DescriptionThe liver is the central metabolic organ. It constantly adapts its metabolic capacity to current physiological requirements. However, the relationship between tissue structure and hepatic function is incompletely understood; this results in a lack of diagnostic markers in medical imaging that can provide information about the liver’s metabolic capacity. Therefore, using normal rabbit livers, we combined magnetic resonance elastography (MRE) with proteomics-based kinetic modeling of central liver metabolism to investigate the potential role of MRE for predicting the liver’s metabolic function in vivo. Nineteen New Zealand white rabbits were investigated by multifrequency MRE and positron-emission tomography (PET). This yielded maps of shear wave speed (SWS), penetration rate (PR), and standardized uptake value (SUV). Proteomic analysis was performed after the scans. Hepatic metabolic functions were assessed on the basis of the HEPATOKIN1 model in combination with a model of hepatic lipid-droplet metabolism using liquid chromatography–mass spectrometry. Our results showed marked differences between individual livers in both metabolic functions and stiffness properties, though not in SUV. When livers were divided into ‘stiff’ and ‘soft’ subgroups (cutoff SWS = 1.6 m/s), stiff livers showed a lower capacity for triacylglycerol storage, while at the same time showing an increased capacity for gluconeogenesis and cholesterol synthesis. Furthermore, SWS was correlated with gluconeogenesis and PR with urea production and glutamine exchange. In conclusion, our study indicates a close relationship between the viscoelastic properties of the liver and metabolic function. This could be used in future studies to predict non-invasively the functional reserve capacity of the liver in patients.
HostingRepositoryPRIDE
AnnounceDate2022-12-21
AnnouncementXMLSubmission_2022-12-21_03:53:34.645.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDavidMeierhofer
SpeciesList scientific name: Oryctolagus cuniculus (Rabbit); NCBI TaxID: 9986;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-09-12 06:22:04ID requested
12022-12-21 03:53:35announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: tomoelastography, PET, proteomics, hepatic function model, reserve capacity, viscoelasticity,Liver metabolism, magnetic resonance elastography, stiffness, MRE
Contact List
IngolfSack
contact affiliationDepartment of Radiology Charité - Universitätsmedizin Berlin Charitéplatz 1 10117 Berlin Germany
contact emailingolf.sack@charite.de
lab head
DavidMeierhofer
contact affiliationMass Spectrometry Facility MPIMG
contact emailmeierhof@molgen.mpg.de
dataset submitter
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Dataset FTP location
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