PXD036612 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Degradomic identification of membrane type 1-matrix metalloproteinase (MT1-MMP/MMP14) as an ADAMTS9 and ADAMTS20 substrate |
Description | ADAMTS9 and ADAMTS20 are homologous secreted proteases implicated in ECM proteolysis and ciliogenesis, but few relevant substrates of these proteases are currently known. Quantitative N-terminomics comparison of RPE-1 cells lacking ADAMTS9 with parental RPE-1 cells identified transmembrane protease MT1-MMP (MMP14) as a novel ADAMTS9 substrate. The resulting enhanced cell-surface MT1-MMP activity in the gene-edited cells contributes to their adhesion defect, but not lack of cilia. A key physiological function of ADAMTS9/20 may be to dampen cell-surface MT1-MMP activity. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_09:02:22.391.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD036612 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Daniel Martin |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | Dimethyl; iTRAQ8plex-116 reporter+balance reagent acylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-09-12 03:40:29 | ID requested | |
1 | 2023-07-20 10:38:12 | announced | |
⏵ 2 | 2023-11-14 09:02:24 | announced | 2023-11-14: Updated project metadata. |
Publication List
10.6019/PXD036612; |
Nandadasa S, Martin D, Deshpande G, Robert KL, Stack MS, Itoh Y, Apte SS, Degradomic Identification of Membrane Type 1-Matrix Metalloproteinase as an ADAMTS9 and ADAMTS20 Substrate. Mol Cell Proteomics, 22(6):100566(2023) [pubmed] |
Keyword List
submitter keyword: Mt1-MMP, RPE-1 cells, ciliogenesis,ADAMTS9, ADAMTS20, MMP14, extracellular matrix |
Contact List
Suneel Apte |
contact affiliation | Department of Biomedical engineering, Lerner Research Institute, Cleveland Clinic |
contact email | aptes@ccf.org |
lab head | |
Daniel Martin |
contact affiliation | Cleveland Clinic |
contact email | danmartin1008@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD036612
- Label: PRIDE project
- Name: Degradomic identification of membrane type 1-matrix metalloproteinase (MT1-MMP/MMP14) as an ADAMTS9 and ADAMTS20 substrate