PXD036612

PXD036612 is an original dataset announced via ProteomeXchange.

Title	Degradomic identification of membrane type 1-matrix metalloproteinase (MT1-MMP/MMP14) as an ADAMTS9 and ADAMTS20 substrate
Description	ADAMTS9 and ADAMTS20 are homologous secreted proteases implicated in ECM proteolysis and ciliogenesis, but few relevant substrates of these proteases are currently known. Quantitative N-terminomics comparison of RPE-1 cells lacking ADAMTS9 with parental RPE-1 cells identified transmembrane protease MT1-MMP (MMP14) as a novel ADAMTS9 substrate. The resulting enhanced cell-surface MT1-MMP activity in the gene-edited cells contributes to their adhesion defect, but not lack of cilia. A key physiological function of ADAMTS9/20 may be to dampen cell-surface MT1-MMP activity.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:02:22.391.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD036612
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Daniel Martin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Dimethyl; iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-12 03:40:29	ID requested
1	2023-07-20 10:38:12	announced
⏵ 2	2023-11-14 09:02:24	announced	2023-11-14: Updated project metadata.

10.6019/PXD036612;

Nandadasa S, Martin D, Deshpande G, Robert KL, Stack MS, Itoh Y, Apte SS, Degradomic Identification of Membrane Type 1-Matrix Metalloproteinase as an ADAMTS9 and ADAMTS20 Substrate. Mol Cell Proteomics, 22(6):100566(2023) [pubmed]

submitter keyword: Mt1-MMP, RPE-1 cells, ciliogenesis,ADAMTS9, ADAMTS20, MMP14, extracellular matrix

Suneel Apte
contact affiliation	Department of Biomedical engineering, Lerner Research Institute, Cleveland Clinic
contact email	aptes@ccf.org
lab head
Daniel Martin
contact affiliation	Cleveland Clinic
contact email	danmartin1008@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD036612

PRIDE project URI

• PRIDE
  1. PXD036612
     1. Label: PRIDE project
     2. Name: Degradomic identification of membrane type 1-matrix metalloproteinase (MT1-MMP/MMP14) as an ADAMTS9 and ADAMTS20 substrate