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PXD036511-1

PXD036511 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleER-Golgi membrane contact sites control the formation of plasma membrane lipid nanodomains required for anthrax intoxication
DescriptionTo promote infections, pathogens exploit host cell machineries including structural elements of the plasma membrane. Studying these interactions and identifying molecular players is an ideal way to gain insights into the fundamental biology of the host cell. Here, we used the anthrax toxin to screen a library of 1500 regulatory, cell surface, and membrane trafficking genes for their involvement in the intoxication process. We found that ER-Golgi localized proteins TMED2 and TMED10 are required for toxin oligomerization at the plasma-membrane of human cells, an essential step dependant on localization to cholesterol-rich lipid nanodomains. Biochemical, morphological and mechanistic analyses showed that TMED2 and TMED10 are essential components of a complex that operates the exchange of both cholesterol and ceramides at ER-Golgi membrane contact sites. Overall, this study of anthrax intoxication led to the discovery that lipid compositional remodelling at ER-Golgi interfaces fully controls the formation of functional membrane nanodomains at the cell surface.
HostingRepositoryPRIDE
AnnounceDate2022-09-07
AnnouncementXMLSubmission_2022-09-07_12:04:06.050.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMuhammad Umair Anwar
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-09-05 09:09:20ID requested
12022-09-07 12:04:06announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: p24 family, TMED2, TMED10, nanodomains, anthrax toxin, membrane contact sites, OSBP, VAPA
Contact List
F. Gisou van der Goot
contact affiliationGlobal Health Institute, School of Life Sciences, EPFL, 1015 Lausanne, Switzerland
contact emailgisou.vandergoot@epfl.ch
lab head
Muhammad Umair Anwar
contact affiliationÉcole Polytechnique Fédérale de Lausanne
contact emailmuhammad.anwar@epfl.ch
dataset submitter
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Dataset FTP location
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