PXD036511

PXD036511 is an original dataset announced via ProteomeXchange.

Title	ER-Golgi membrane contact sites control the formation of plasma membrane lipid nanodomains required for anthrax intoxication
Description	To promote infections, pathogens exploit host cell machineries including structural elements of the plasma membrane. Studying these interactions and identifying molecular players is an ideal way to gain insights into the fundamental biology of the host cell. Here, we used the anthrax toxin to screen a library of 1500 regulatory, cell surface, and membrane trafficking genes for their involvement in the intoxication process. We found that ER-Golgi localized proteins TMED2 and TMED10 are required for toxin oligomerization at the plasma-membrane of human cells, an essential step dependant on localization to cholesterol-rich lipid nanodomains. Biochemical, morphological and mechanistic analyses showed that TMED2 and TMED10 are essential components of a complex that operates the exchange of both cholesterol and ceramides at ER-Golgi membrane contact sites. Overall, this study of anthrax intoxication led to the discovery that lipid compositional remodelling at ER-Golgi interfaces fully controls the formation of functional membrane nanodomains at the cell surface.
HostingRepository	PRIDE
AnnounceDate	2022-09-07
AnnouncementXML	Submission_2022-09-07_12:04:06.050.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Muhammad Umair Anwar
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-05 09:09:20	ID requested
⏵ 1	2022-09-07 12:04:06	announced

Dataset with its publication pending

submitter keyword: p24 family, TMED2, TMED10, nanodomains, anthrax toxin, membrane contact sites, OSBP, VAPA

F. Gisou van der Goot
contact affiliation	Global Health Institute, School of Life Sciences, EPFL, 1015 Lausanne, Switzerland
contact email	gisou.vandergoot@epfl.ch
lab head
Muhammad Umair Anwar
contact affiliation	École Polytechnique Fédérale de Lausanne
contact email	muhammad.anwar@epfl.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD036511

PRIDE project URI

• PRIDE
  1. PXD036511
     1. Label: PRIDE project
     2. Name: ER-Golgi membrane contact sites control the formation of plasma membrane lipid nanodomains required for anthrax intoxication