PXD036470

PXD036470 is an original dataset announced via ProteomeXchange.

Title	Proteomic, transcriptomic, biochemical, and physio-anatomical analyses provide insights into energy metabolism, light utilization, and photosynthesis in cigar tobacco under different light intensities
Description	Low light (LL) or shading is detrimental one among other abiotic stresses that limit plant growth and suppress the crop plants' productivity. However, shading is one of the key cultivation techniques in cigar wrapper tobacco production. A gap of knowledge is present related to transcriptional and translational regulatory networks under such conditions. Herein, this work is planned for the integrative profiling of transcriptome and proteome along with some biochemical and physio-anatomical analyses under different light intensities [T200 (200 μmol m−2 s−1), T100 (100 μmol m−2 s−1), and T50 (50 μmol m−2 s−1)] to uncover the underlying molecular response mechanisms. The result showed that the leaf anatomical structure has a relationship with leaf photosynthetic capacity; as LL intensities (particularly T50) decreased the leaf thickness and thickness of palisade, and spongy tissues resulting in a lower photosynthesis rate and eventually decreased the plant biomass compared with T200. Furthermore, 3045 differentially expressed genes (DEGs) and 966 differentially expressed proteins (DEPs) were identified in the transcriptome and proteome of cigar tobacco. Furthermore, the integrative analysis between both omics revealed a total of 160 correlated DEGs and DEPs. The integrated analysis of both omics showed that the shared DEGs and DEPs were upregulated in photosynthesis-antenna proteins, photosynthesis, and defense/detoxification-related pathways. While the correlated DEGs and DEPs were downregulated in glycolysis, starch and sucrose metabolism, tyrosine metabolism, and mitochondrial electron transport chain pathways along with decreased activities of glyceraldehyde-3-phosphate dehydrogenase, starch phosphorylase, and pyruvate kinase enzymes related to glycolysis. These results showed that cigar tobacco efficiently utilized low light and reconfigured its energy metabolism. Collectively, this study can offer profound insights into the response mechanism at the physio-anatomical, biochemical, and molecular levels and showcases valuable resources of genes and proteins for future functional studies underlying LL response.
HostingRepository	iProX
AnnounceDate	2022-09-01
AnnouncementXML	Submission_2022-09-02_06:28:22.215.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xinghua Ma
SpeciesList	scientific name: Nicotiana tabacum; NCBI TaxID: 4097;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-02 06:27:53	ID requested
⏵ 1	2022-09-02 06:28:22	announced

Dataset with its publication pending

submitter keyword: Cigar Tobacco, Low light, Light intensities, Shading, Transcriptome and Proteome

Dingchun Yan
contact affiliation	Tobacco Research Institute of Chinese Academy of Agricultural Sciences
contact email	yandingchun@caas.cn
lab head
Xinghua Ma
contact affiliation	Tobacco Research Institute of Chinese Academy of Agricultural Sciences
contact email	maxinghua@caas.cn
dataset submitter

iProX dataset URI