Low light (LL) or shading is detrimental one among other abiotic stresses that limit plant growth and suppress the crop plants' productivity. However, shading is one of the key cultivation techniques in cigar wrapper tobacco production. A gap of knowledge is present related to transcriptional and translational regulatory networks under such conditions. Herein, this work is planned for the integrative profiling of transcriptome and proteome along with some biochemical and physio-anatomical analyses under different light intensities [T200 (200 μmol m−2 s−1), T100 (100 μmol m−2 s−1), and T50 (50 μmol m−2 s−1)] to uncover the underlying molecular response mechanisms. The result showed that the leaf anatomical structure has a relationship with leaf photosynthetic capacity; as LL intensities (particularly T50) decreased the leaf thickness and thickness of palisade, and spongy tissues resulting in a lower photosynthesis rate and eventually decreased the plant biomass compared with T200. Furthermore, 3045 differentially expressed genes (DEGs) and 966 differentially expressed proteins (DEPs) were identified in the transcriptome and proteome of cigar tobacco. Furthermore, the integrative analysis between both omics revealed a total of 160 correlated DEGs and DEPs. The integrated analysis of both omics showed that the shared DEGs and DEPs were upregulated in photosynthesis-antenna proteins, photosynthesis, and defense/detoxification-related pathways. While the correlated DEGs and DEPs were downregulated in glycolysis, starch and sucrose metabolism, tyrosine metabolism, and mitochondrial electron transport chain pathways along with decreased activities of glyceraldehyde-3-phosphate dehydrogenase, starch phosphorylase, and pyruvate kinase enzymes related to glycolysis. These results showed that cigar tobacco efficiently utilized low light and reconfigured its energy metabolism. Collectively, this study can offer profound insights into the response mechanism at the physio-anatomical, biochemical, and molecular levels and showcases valuable resources of genes and proteins for future functional studies underlying LL response.