<<< Full experiment listing

PXD036450-1

PXD036450 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDiadenosine tetraphosphate as a cosubstrate for AMPylation mediated by FICD
DescriptionDiadenosine polyphosphates (ApnAs) are noncanonical nucleotides that are ubiquitous across all forms of life. Due to their strong accumulation upon cellular stress, they are considered alarmones to signal homeostatic imbalance. Nonetheless, their cellular role is poorly understood. In this work, we assessed ApnAs for their usage as cosubstrate for protein AMPylation, a post-translational modification. In humans, AMPylation mediated by the AMPylator FICD with ATP as cosubstrate is a response to ER stress. Here, we demonstrate that Ap4A is proficiently consumed for AMPylation by FICD. By chemical proteomics using a new chemical probe, we identified new potential AMPylation targets. Interestingly, we found that AMPylation targets of FICD may differ depending on the nucleotide cosubstrate. These results may suggest that signaling at elevated Ap4A levels during cellular stress differs from when Ap4A is present at low concentrations allowing response to extracellular cues.
HostingRepositoryPRIDE
AnnounceDate2023-01-27
AnnouncementXMLSubmission_2023-01-27_00:25:48.178.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPhilipSaumer
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; adenylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-09-02 04:56:06ID requested
12023-01-27 00:25:48announced
22023-11-14 08:58:05announced2023-11-14: Updated project metadata.
Publication List
Keyword List
submitter keyword: Diadenosine tetraphosphate, AMPylation, FICD, Post-translational modification, Proteomics
Contact List
AndreasMarx
contact affiliationOrganic Chemistry / Cellular Chemistry, Dept. of Chemistry, University of Konstanz (Germany)
contact emailandreas.marx@uni-konstanz.de
lab head
PhilipSaumer
contact affiliationUniversity of Konstanz
contact emailphilip.saumer@uni-konstanz.de
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/01/PXD036450
PRIDE project URI
Repository Record List
[ + ]