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PXD036446-1

PXD036446 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHDX-MS of AcrIF9 and AcrIF2 binding to CRISPR IF Csy complex
DescriptionCRISPR RNA-guided detection and degradation of foreign DNA is a dynamic process. Viruses can interfere with this cellular defense by expressing small proteins called anti-CRISPRs. While structural models of anti-CRISPRs bound to their target complex provide static snapshots that inform mechanism, the dynamics and thermodynamics of these interactions are often overlooked. Here we use hydrogen deuterium exchange-mass spectrometry (HDX-MS) and differential scanning fluorimetry (DSF) experiments to determine how anti-CRISPR binding impacts the conformational landscape of the type IF CRISPR RNA guided surveillance complex (Csy) upon binding of two different anti-CRISPR proteins (AcrIF9 and AcrIF2). The results demonstrate that AcrIF2 binding relies on enthalpic stabilization, whereas AcrIF9 uses an entropy driven reaction to bind the CRISPR RNA-guided surveillance complex. Collectively, this work reveals the thermodynamic basis and mechanistic versatility of anti-CRISPR-mediated immune suppression. More broadly, this work presents a striking example of how allosteric effectors are employed to regulate nucleoprotein complexes.
HostingRepositoryPRIDE
AnnounceDate2022-11-01
AnnouncementXMLSubmission_2022-11-01_06:58:00.240.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAngelaPatterson
SpeciesList scientific name: Pseudomonas aeruginosa N002; NCBI TaxID: 1194669;
ModificationListNo PTMs are included in the dataset
InstrumentAgilent instrument model; Bruker Daltonics micrOTOF series
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-09-01 02:10:37ID requested
12022-11-01 06:58:00announced
22023-11-14 08:59:06announced2023-11-14: Updated project metadata.
Publication List
Keyword List
submitter keyword: Csy,HDX, Acr
Contact List
BrianBothner
contact affiliationDepartment of Chemistry and Biochemistry Montana State University
contact emailbbothner@montana.edu
lab head
AngelaPatterson
contact affiliationIndiana University (Bloomington, IN)
contact emailap4@iusb.edu
dataset submitter
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Dataset FTP location
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