PXD036356 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Grass carp SERPINA1 CoIP with hepatopancreas mass spectrometry analysis |
Description | SERPINA1, a member of the serine protease inhibitor family, plays a role in viral infection and inflammation by regulating the activities of serine and cysteine proteases. To further investigate the antiviral role SERPINA1 played in GCRV (Grass carp Reovirus) infection, a polyclonal antibody of SERPINA1 was prepared, and the protein interacting with SERPINA1 was screened by CoIP/MS in grass carp hepatopancreas tissue. Samples of hepatopancreas tissues from grass carp (n=3) before (healthy) and 8 days after the infection (post-infection) were selected. Grass carp were infected by intraperitoneal injection. The total tissue proteins were extracted according to the manufacturer’s instructions for cell lysate (Beyotime, Shanghai, China). The types and abundance of proteins bound to SERPINA1 before and after the infection were detected and analyzed using CoIP-MS. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:58:03.843.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yangyang Li |
SpeciesList | scientific name: Ctenopharyngodon idella; NCBI TaxID: 7959; |
ModificationList | No PTMs are included in the dataset |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-08-27 11:31:51 | ID requested | |
1 | 2022-08-30 17:40:40 | announced | |
⏵ 2 | 2023-11-14 08:58:04 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: SERPINA1,grass carp, CoIP/MS, hepatopancreas |
Contact List
Yaping Wang |
contact affiliation | State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences (CAS), China. |
contact email | wangyp@ihb.ac.cn |
lab head | |
Yangyang Li |
contact affiliation | State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China |
contact email | lyywh613@hotmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD036356
- Label: PRIDE project
- Name: Grass carp SERPINA1 CoIP with hepatopancreas mass spectrometry analysis