PXD036356

PXD036356 is an original dataset announced via ProteomeXchange.

Title	Grass carp SERPINA1 CoIP with hepatopancreas mass spectrometry analysis
Description	SERPINA1, a member of the serine protease inhibitor family, plays a role in viral infection and inflammation by regulating the activities of serine and cysteine proteases. To further investigate the antiviral role SERPINA1 played in GCRV (Grass carp Reovirus) infection, a polyclonal antibody of SERPINA1 was prepared, and the protein interacting with SERPINA1 was screened by CoIP/MS in grass carp hepatopancreas tissue. Samples of hepatopancreas tissues from grass carp (n=3) before (healthy) and 8 days after the infection (post-infection) were selected. Grass carp were infected by intraperitoneal injection. The total tissue proteins were extracted according to the manufacturer’s instructions for cell lysate (Beyotime, Shanghai, China). The types and abundance of proteins bound to SERPINA1 before and after the infection were detected and analyzed using CoIP-MS.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:58:03.843.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yangyang Li
SpeciesList	scientific name: Ctenopharyngodon idella; NCBI TaxID: 7959;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-27 11:31:51	ID requested
1	2022-08-30 17:40:40	announced
⏵ 2	2023-11-14 08:58:04	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: SERPINA1,grass carp, CoIP/MS, hepatopancreas

Yaping Wang
contact affiliation	State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences (CAS), China.
contact email	wangyp@ihb.ac.cn
lab head
Yangyang Li
contact affiliation	State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
contact email	lyywh613@hotmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD036356

PRIDE project URI

• PRIDE
  1. PXD036356
     1. Label: PRIDE project
     2. Name: Grass carp SERPINA1 CoIP with hepatopancreas mass spectrometry analysis