PXD036335 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A proteome-wide effect of PHF8 knockdown in neuronal activity-dependent synaptic plasticity shows downregulation of Parkinson's disease-associated protein Alpha-synuclein and its interactors |
Description | Synaptic dysfunction may underlie the pathophysiology of Parkinson’s disease (PD): a presently incurable condition characterized by motor and cognitive symptoms. Here we used quantitative proteomics to study the role of PHF8, a chromatin-modifying enzyme found to be mutated in intellectual disability, in regulating the expression of synaptic plasticity-related proteins, amongst which the Parkinson’s disease-associated alpha synuclein (SNCA) and its interactors including CamKIIa, CPLX1, RPH3A, CALB1, CHN1 were prominently featured. Depletion of PHF8 in cortical neurons affected the activity-induced expression of proteins involved in synaptic plasticity, synaptic structure, vesicular release and membrane trafficking, spanning the spectrum of pre-synaptic and post-synaptic transmission. By showing how the depletion of one chromatin-modifying enzyme can affect synaptic function in a concerted manner, we propose the possibility of targeting PHF8 as a potential disease-modifying therapeutic drug target in PD. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:25:59.014.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Lei Zhou |
SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; |
ModificationList | No PTMs are included in the dataset |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-08-26 07:55:04 | ID requested | |
1 | 2023-03-10 13:51:07 | announced | |
⏵ 2 | 2023-11-14 08:25:59 | announced | 2023-11-14: Updated project metadata. |
Publication List
Oey NE, Zhou L, Chan CHS, VanDongen AMJ, Tan EK, A Proteome-Wide Effect of PHF8 Knockdown on Cortical Neurons Shows Downregulation of Parkinson's Disease-Associated Protein Alpha-Synuclein and Its Interactors. Biomedicines, 11(2):(2023) [pubmed] |
Keyword List
submitter keyword: synaptic plasticity, Neurodegeneracy,Proteomics, Parkinson's disease, neurodegeneration |
Contact List
Lei ZHOU |
contact affiliation | Singapore Eye Research Institute |
contact email | zhou.lei.seri@gmail.com |
lab head | |
Lei Zhou |
contact affiliation | Singapore Eye Research Institute |
contact email | zhou.lei@seri.com.sg |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD036335 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD036335
- Label: PRIDE project
- Name: A proteome-wide effect of PHF8 knockdown in neuronal activity-dependent synaptic plasticity shows downregulation of Parkinson's disease-associated protein Alpha-synuclein and its interactors