PXD036335

PXD036335 is an original dataset announced via ProteomeXchange.

Title	A proteome-wide effect of PHF8 knockdown in neuronal activity-dependent synaptic plasticity shows downregulation of Parkinson's disease-associated protein Alpha-synuclein and its interactors
Description	Synaptic dysfunction may underlie the pathophysiology of Parkinson’s disease (PD): a presently incurable condition characterized by motor and cognitive symptoms. Here we used quantitative proteomics to study the role of PHF8, a chromatin-modifying enzyme found to be mutated in intellectual disability, in regulating the expression of synaptic plasticity-related proteins, amongst which the Parkinson’s disease-associated alpha synuclein (SNCA) and its interactors including CamKIIa, CPLX1, RPH3A, CALB1, CHN1 were prominently featured. Depletion of PHF8 in cortical neurons affected the activity-induced expression of proteins involved in synaptic plasticity, synaptic structure, vesicular release and membrane trafficking, spanning the spectrum of pre-synaptic and post-synaptic transmission. By showing how the depletion of one chromatin-modifying enzyme can affect synaptic function in a concerted manner, we propose the possibility of targeting PHF8 as a potential disease-modifying therapeutic drug target in PD.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:25:59.014.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lei Zhou
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-26 07:55:04	ID requested
1	2023-03-10 13:51:07	announced
⏵ 2	2023-11-14 08:25:59	announced	2023-11-14: Updated project metadata.

Oey NE, Zhou L, Chan CHS, VanDongen AMJ, Tan EK, A Proteome-Wide Effect of PHF8 Knockdown on Cortical Neurons Shows Downregulation of Parkinson's Disease-Associated Protein Alpha-Synuclein and Its Interactors. Biomedicines, 11(2):(2023) [pubmed]

submitter keyword: synaptic plasticity, Neurodegeneracy,Proteomics, Parkinson's disease, neurodegeneration

Lei ZHOU
contact affiliation	Singapore Eye Research Institute
contact email	zhou.lei.seri@gmail.com
lab head
Lei Zhou
contact affiliation	Singapore Eye Research Institute
contact email	zhou.lei@seri.com.sg
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD036335

PRIDE project URI

• PRIDE
  1. PXD036335
     1. Label: PRIDE project
     2. Name: A proteome-wide effect of PHF8 knockdown in neuronal activity-dependent synaptic plasticity shows downregulation of Parkinson's disease-associated protein Alpha-synuclein and its interactors