PXD036326

PXD036326 is an original dataset announced via ProteomeXchange.

Title	Arterial-Venous Endothelial Cell Cycle Proteins Differential Expression
Description	HUVEC-FUCCI cells were used to demonstrate that different endothelial cell cycle states provide distict windows of opportunity for gene expression in response to extrinsic signals. HUVEC-FUCCI were FACS-isolated into three different cell cycle states. Peptide digests from the resulting lysates showed differentially expressed proteins among the three cell cycles. These studies show that endothelial cell cycle state determines the propensity for arterial vs. venous fate specification.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:29:31.575.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Erin Jeffery
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-26 07:29:30	ID requested
1	2022-10-25 06:31:09	announced
2	2023-11-14 07:32:41	announced	2023-11-14: Updated project metadata.
⏵ 3	2023-11-14 08:29:38	announced	2023-11-14: Updated project metadata.

Chavkin NW, Genet G, Poulet M, Jeffery ED, Marziano C, Genet N, Vasavada H, Nelson EA, Acharya BR, Kour A, Aragon J, McDonnell SP, Huba M, Sheynkman GM, Walsh K, Hirschi KK, Endothelial cell cycle state determines propensity for arterial-venous fate. Nat Commun, 13(1):5891(2022) [pubmed]

Laufs U, La Fata V, Plutzky J, Liao JK, Upregulation of endothelial nitric oxide synthase by HMG CoA reductase inhibitors. Circulation, 97(12):1129-35(1998) [pubmed]

submitter keyword: proteomics, proteogenomics, early G1, S/G2/M, endothelial cells,HUVEC, late G1, cell cycle

Dr. Gloria Sheynkman
contact affiliation	Department of Molecular Physiology and Biological Physics University of Virginia School of Medicine Charlottesville, VA, USA 22908
contact email	gs9yr@virginia.edu
lab head
Erin Jeffery
contact affiliation	University of Virginia, Department of Molecular Physiology and Biological Physics
contact email	edf4n@virginia.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD036326

PRIDE project URI

• PRIDE
  1. PXD036326
     1. Label: PRIDE project
     2. Name: Arterial-Venous Endothelial Cell Cycle Proteins Differential Expression