PXD036285 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | TraB is a component of the ER-Mitochondrial contact site regulating ER-Mitochondrial interaction and mitophagy |
Description | ER-mitochondria contact sites (EMCSs) are important for mitochondrial function. Here, we have identified a EMCS complex, comprising a family of uncharacterised mitochondrial outer- membrane proteins, TraB1, TraB2 and the ER protein, VAP27-1. In Arabidopsis, there are three TraB isoforms and the trab1a/trab2 double mutant exhibits abnormal mitochondrial morphology, strong starch accumulation and impaired energy metabolism, indicating that these proteins are essential for normal mitochondrial function. Moreover, TraB1 and TraB2 proteins also interact with ATG8 in order to regulate mitochondrial degradation (mitophagy). The turnover of depolarised mitochondria is significantly reduced in both trab1/trab2 and VAP27 mutants (vap27-1,3,4,6) under mitochondrial stress conditions, with an increased population of dysfunctional mitochondria present in the cytoplasm. Consequently, plant recovery after stress is significantly perturbed, suggesting that TraB1 regulated mitophagy and ER-mitochondrial interaction are two closely related processes. Taken together, we ascribe a dual role to TraB1 which is a component of the EMCS complex in eukaryotes, regulating both interaction of the mitochondria to the ER and mitophagy. |
HostingRepository | PRIDE |
AnnounceDate | 2022-08-30 |
AnnouncementXML | Submission_2022-08-30_02:07:19.554.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD036285 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Dominique Eeckhout |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-08-25 13:50:44 | ID requested | |
⏵ 1 | 2022-08-30 02:07:19 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: TraB / VAP27 / TAP / Membrane contact sites / Endoplasmic Reticulum / Mitochondria / Autophagy / Mitophagy / Arabidopsis |
Contact List
Pengwei Wang |
contact affiliation | Key Laboratory of Horticultural Plant Biology (MOE), Huazhong Agricultural University, Wuhan, 430070, China Hubei Hongshan Laboratory, Wuhan, 430070, China |
contact email | wangpengwei@mail.hzau.edu.cn |
lab head | |
Dominique Eeckhout |
contact affiliation | Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, B-9052, Belgium VIB Center for Plant Systems Biology, Ghent, B-9052, Belgium |
contact email | Dominique.Eeckhout@psb.ugent.be |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD036285
- Label: PRIDE project
- Name: TraB is a component of the ER-Mitochondrial contact site regulating ER-Mitochondrial interaction and mitophagy