PXD036232

PXD036232 is an original dataset announced via ProteomeXchange.

Title	MHCI peptidome of HepG2 with ArgRS or SRRM2 knock down
Description	Cells respond to perturbations like inflammation by sensing changes in metabolite levels. Especially prominent is arginine, which has known connections to the inflammatory response. Here, we found that depletion of arginine during inflammation decreased levels of a nuclear form of arginyl-tRNA synthetase (ArgRS). Surprisingly, we found that nuclear ArgRS interacts and co-localizes with serine/arginine repetitive matrix protein 2 (SRRM2), a spliceosomal and nuclear speckle protein, and that decreased levels of nuclear ArgRS correlated with changes in condensate-like nuclear trafficking of SRRM2 and splice-site usage in certain genes. These splice-site usage changes cumulated in the synthesis of different protein isoforms that altered cellular metabolism and peptide presentation to immune cells. Our findings uncover a novel mechanism whereby a tRNA synthetase cognate to a key amino acid that is metabolically controlled during inflammation modulates the splicing machinery.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:57:49.265.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD036232
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Haissi Cui
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-22 04:02:45	ID requested
1	2023-04-14 12:25:40	announced
⏵ 2	2023-11-14 08:57:49	announced	2023-11-14: Updated project metadata.

10.6019/PXD036232;

submitter keyword: inflammation,MHCI, peptidome

Paul Schimmel
contact affiliation	Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
contact email	schimmel@scripps.edu
lab head
Haissi Cui
contact affiliation	Scripps Research
contact email	hacui@scripps.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/04/PXD036232

PRIDE project URI

• PRIDE
  1. PXD036232
     1. Label: PRIDE project
     2. Name: MHCI peptidome of HepG2 with ArgRS or SRRM2 knock down