PXD036162-1
PXD036162 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | LKB1/STK11-sensing of mitochondrial function controls Th17 cell responses |
Description | T helper 17 (Th17) cells are a distinct subset of CD4+ T cells necessary for maintaining gut homeostasis and have prominent roles in autoimmunity and inflammation. Th17 cells have unique metabolic features, including a stem cell-like signature and reliance on mitochondrial respiratory chain function and tricarboxylic acid (TCA) cycle to coordinate metabolic and epigenetic remodeling. Dynamic changes in mitochondrial membrane morphology are key to sustain organelle function. However, it remains unclear whether mitochondrial membrane remodeling orchestrates metabolic and differentiation events in Th17 cells. Here we demonstrate that mitochondrial membrane fusion and tight cristae organization are required for Th17 cell function (i.e. cytokine expression). As a genetic model system we employ Th17 specific deletion of optic atrophy 1 (OPA1), a gene that encodes a protein involved in mitochondrial inner membrane fusion and cristae organization. As a result, we find that Th17 cells rely on mitochondrial fusion (due to their low metabolic activity). Here, we carry out DIA-based differential quantitative proteomic analysis of murine wild-type and OPA1 knock-out Th17 cells. Through Ingenuity pathway analysis and together with transcriptional and metabolomic profiling we identify the serine/threonine kinase liver associated kinase B1 (LKB1/STK11) as an essential node coupling mitochondrial function to IL-17A cytokine expression in T cells. |
HostingRepository | PRIDE |
AnnounceDate | 2022-10-13 |
AnnouncementXML | Submission_2022-10-13_11:08:04.300.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | GerhardMittler |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2022-08-19 00:36:48 | ID requested | |
⏵ 1 | 2022-10-13 11:08:05 | announced | |
2 | 2023-11-14 08:28:11 | announced | 2023-11-14: Updated project metadata. |
Publication List
Baixauli F, Piletic K, Puleston DJ, Villa M, Field CS, Flachsmann LJ, Quintana A, Rana N, Edwards-Hicks J, Matsushita M, Stanczak MA, Grzes KM, Kabat AM, Fabri M, Caputa G, Kelly B, Corrado M, Musa Y, Duda KJ, Mittler G, O'Sullivan D, Sesaki H, Jenuwein T, Buescher JM, Pearce EJ, Sanin DE, Pearce EL, 17 effector function. Nature, 610(7932):555-561(2022) [pubmed] |
Keyword List
submitter keyword: DIA, Spectronaut,Th17 T cells, serine/threonine kinase liver associated kinase B1 (LKB1/STK11)., OPA1 knock out Th17 cells |
Contact List
GerhardMittler | |
---|---|
contact affiliation | Proteomics Unit Max Planck Institute of Immunobiology and Epigenetics |
contact email | mittler@ie-freiburg.mpg.de |
lab head | |
GerhardMittler | |
contact affiliation | Proteomics Unit, Max Planck Institute of Immunobiology and Epigenetics |
contact email | mittler@ie-freiburg.mpg.de |
dataset submitter |
Full Dataset Link List
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PRIDE project URI |
Repository Record List
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