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PXD036148-1

PXD036148 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleComparative Proteomic Analysis of the Mitochondria of Menstrual Stem Cells and Ovarian Cancer Cells
DescriptionMitochondria transplantation is a hot spot in cell-free therapy. Menstrual stem cells (MenSCs) are potential donor cells to provide foreign mitochondria. This study aims to investigate the possible effects of MenSC-derived mitochondria on ovarian cancer from the perspective of protein expression profiling. MenSCs were harvested from menstrual blood. The mitochondria were isolated from MenSCs and ovarian cancer cell line-SKOV3. A label-free proteomics and analysis were performed by comparing the protein expression in the mitochondria of MenSCs and SKOV3 cells. The differentially expressed proteins with fold change higher than 2 were analyzed by Gene Ontology, KEGG pathway and protein domain enrichment analysis, protein interaction networks and PRM analysis. In total, 592 proteins which were up-expressed in the mitochondria of MenSCs were taken to be analyzed. Functional enrichment analysis revealed these proteins were enriched in metabolism-related pathway entries including oxidative phosphorylation (OXPHOS) pathway. Parallel Reaction Monitoring (PRM) analysis confirmed that 4 out of 6 candidate proteins on OXPHOS pathway afforded similar trends. The protein domain enrichment analysis showed that the domains such as thioredoxin domain were significantly enriched. Based on these findings, we believed that the mitochondria from MenSCs might work to enhance the progression of ovarian cancer, which was probably mediated by the enrichment of OXPHOS-related metabolic pathways.
HostingRepositoryPRIDE
AnnounceDate2022-10-12
AnnouncementXMLSubmission_2022-10-12_09:37:59.642.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterwenzhong
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentMaxQuant
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-08-18 07:54:46ID requested
12022-10-12 09:37:59announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Energy metabolism, Menstrual stem cell, Mitochondria transplantation, OXPHOS, Proteomics
Contact List
XiuhuiChen
contact affiliationDepartment of obstetrics and gynecology. Second affiliated hospital of Harbin Medical University. Harbin, Heilongjiang, China.
contact email106942878@qq.com
lab head
wenzhong
contact affiliationThe 2nd affiliated hospital of Harbin medical university
contact emaildrzhong96@163.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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